Nanobiotechnology is exhibiting the great potential to be actively utilized in the form of nanoparticles to serve as carriers of biomolecules and benefit human health. In the present work, we have synthesized silver nanoparticles via a green synthesis pathway using medicinal plants Eucalyptus camaldulensis, Azadirachta indica, and Euphorbia heterophylla (a) separately and (b) in combination for synthesis. The major focus of this study is to assess the efficiency of silver nanoparticles when synthesized from a combination of medicinal plant leaf extracts with enhanced efficiency. The formation of silver nanoparticles was analyzed by the color change of the reaction mixture and Ultraviolet-Visible spectroscopic analysis (UV-Vis) showing characteristic peak of silver nanoparticles. Fourier Transform Infra-red (FTIR) analysis confirmed the various types of secondary metabolites playing active role in synthesis of silver nanoparticles alongwith bio-capping of synthesized silver nanoparticles. When compared with other published results, we concluded that silver nanoparticles synthesized from a combination of leaf extracts from medicinal plants yielded better results and were found to be more promising. Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), and X-Ray Diffraction (XRD) provided key results in terms of particle size, hydrodynamic size, shape, and structure. Our study is the first of its kind where medicinal properties of the aforementioned plants were employed to study their impact on the most commonly found bio-fertilizer of the Indian subcontinent; cyanobacteria Anabaena doliolum.
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