Confocal laser-scanning microscopy was chosen to observe the colonization and damage caused by the soft rot Pectobacterium atrosepticum and the protection mediated by the biocontrol agent Rhodococcus erythropolis. We developed dual-color reporter strains suited for monitoring quorum-sensing and quorum-quenching activities leading to maceration or biocontrol, respectively. A constitutively expressed cyan or red fluorescent protein served as a cell tag for plant colonization, while an inducible expression reporter system based on the green fluorescent protein gene enabled the simultaneous recording of signaling molecule production, detection, or degradation. The dual-colored pathogen and biocontrol strains were used to coinoculate potato tubers. At cellular quorum, images revealed a strong pectobacterial quorum-sensing activity, especially at the plant cell walls, as well as a concomitant rhodococcal quorum-quenching response, at both the single-cell and microcolony levels. The generated biosensors appear to be promising and complementary tools useful for molecular and cellular studies of bacterial communication and interference.

Kytococcus schroeteri strain H01 was isolated from the skin of a healthy volunteer who underwent erythromycin treatment for a skin disorder 1 year prior. The draft genome consists of 2.38 Mb, a G+C content of 73.06%, and 2,221 protein coding sequences. This is the first genome characterization of a K. schroeteri strain isolated from human skin.

<p>By 2050, bacterial infections could become the leading cause of death due to the emergence and spread of antimicrobial resistant-bacteria (ARB) and -genes (ARG). Faced to this global problem, an integrative approach (human animal environment) is encouraged by the WHO. However, environmental antimicrobial resistance (AMR) remains insufficiently investigated, with a focus on wastewater and a disregard to current or emerging clinical concerns.</p><p>ARB and ARGs could be found in water intended for human consumption. Like many Mediterranean cities, Montpellier and surroundings (southeastern France) are predominantly supplied by water from karst aquifers that are vulnerable to contaminations above all in climatic changes context. In France, although the microbiological quality of drinking water is strictly monitored, AMR is not. This study is conducted at a local scale to i) test the concept of the use of the regulatory surveillance of drinking water to monitor AMR in a French area and ii) evaluate the dynamics of AMR in drinking water in relation with climatic events.</p><p>From 2024 samples (sanitary surveillance Aude and Hérault) conducted from January to April 2021, 85 <em>Escherichia coli</em> were isolated according to the regulatory protocol and studied for their antimicrobial resistance profile. <em>bla<sub>TEM</sub>, bla<sub>SHV</sub>, bla<sub>CTX-M</sub></em><sub></sub>that are the most frequent beta-lactamases-encoding ARG in human infections were researched in 37 cultivable Gram-negative community. Water was also sampled at the entering of drinking water treatment plant (Montpellier), under contrasted hydroclimatic conditions.</p><p><em>bla<sub>TEM</sub></em> was found in 14% of <em>E. coli</em> while no <em>E. coli</em> carried <em>bla<sub>SHV</sub></em> or <em>bla<sub>CTX-M</sub></em>. <em>bla<sub>TEM</sub>, bla<sub>SHV</sub>, bla<sub>CTX-M</sub></em> were encountered in 43%, 3% and 0% of cultivable Gram-negative community respectively. The <em>E. coli</em> strains carrying the <em>bla<sub>TEM</sub></em> gene and the communities positive for <em>bla<sub>SHV</sub></em><sub></sub>came mainly from karst aquifers.</p><p>Antibiotic resistance in drinking water in France had never been explored on this large scale. We showed the wide diffusion of TEM producing <em>E. coli</em> in drinking water as described in human samples. With this preliminary study we prove that the existing nation-wide regulatory surveillance network could be used with slight modifications to monitor AMR in an environmental compartment that directly expose human populations.</p>