The relationships of 12 species in six genera in the tribe Anthemideae of the Asteraceae such as Achillea santolina, A. fragrantissima, Anacyclus monanthos, Cotula anthemoids, C. barbata, C. cinerea, Glebionis coronaria var. discolor, Matricaria recutita and M. aurea collected in different localities in Egypt and have been addressed based on polymophism in ITS sequence. Phylogenetic relationships were constructed using bioinformatic tools based on molecular data of ITS sequence. Studied taxa showed cryptic differences in ITS and thus, they could be distinctly separated from the other close relatives of the genera in the tribe Anthemideae.