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The objective of this research was to study the feasibility of using roselle calyces and cinnamon extracts as enriching bioactive components in ice milk making. Formulations of ice milk containing 2%,4% roselle extract and 10%,15% cinnamon extract was carried out. The chemical analysis of roselle extract indicated higher total phenolic, total flavonoids, tannic acid, total antioxidant and anthocyanin contents, compared with cinnamon extract. The highest overrun were observed in the control sample and ice milk with roselle calyces extract of 2%. The control samples had the lowest flavonoid, anthocyanin and tannic acid contents, while ice milk samples with roselle extracts 2 and 4% additive ratio were of the greatest concentration of flavonoid, anthocyanin and tannic acid. Phenolic content of most ice milk samples showed slight increases during the storage at -18 o C for 30 days. The total antioxidant content (TAC) increased in the ice milk by increasing the ratio of roselle calyces extracts and cinnamon extract. The differences in sensory scores of ice milk samples were no statistically significant (P>0.05) in the colour, flavour , taste, mouth feel and texture. The total bacterial count of the ice milk formulations was within the safe levels.

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The current research was performed to evaluate the potential protective effect of Lactobacillus paracasei ssp paracasei, Pediococcus acidi-lactis and Lactoccus lactis ssp lactis, Sylimarin in the alleviation of health (hepatic and renal) complications caused by carbon tetrachloride (CCl4) in rats. Healthy sixty albino rats were divided into six groups, the first group was control (negative), the second group (control positive) was injected CCl4 (1 ml/kg, 1:1 v/v paraffin oil mixture, i.p. every third day for 8 weeks, The third group (CCl4 + silymarin group) receiving both CCl4 and daily silymarin therapy (50 mg/kg, oral), the fourth group: CCl4+ (Lactobacillus paracasei 1ml orally). The fifth group (CCl4+ Pediococcus acidilactis 1ml orally) and the six group (CCl4+ Lactococcus lactis (1ml orally) for eight weeks per day. Biochemical markers were tested for blood, liver and kidney tissue. Histopathological tests on liver and kidney tissues were performed. The findings obtained have shown that Lactobacillus paracasei ssp paracasei, Pediococcus acidilactis and Lactoccus lactis ssp lactis improved the disrupted biochemical parameters caused by CCl4 therapy. Besides, the findings of the histopathological analysis are in consistent with biochemical parameters and the protective ability of lactic acid bacteria suggesting that the best lactic acid bacteria was Pediococcus acidilactis that helped strengthen liver fibrosis caused by CCl4 therapy, while the best bacterium for improving renal damage was Lactoccus lactis.