AIM: This study investigated the effect of different finishing and polishing systems on surface roughness of two types of universal nanohybrid composite resins. MATERIALS AND METHODS: A total number of 40 samples will be prepared to form two main equal groups of specimens (n = 20), according to the composite resin materials. Two universal nanohybrid resin composites were used in this study. First group (A1) is a universal nanohybrid composite resin (Mosaic) and the second group (A2) is a universal nanohybrid composite resin (Harmonize). Each group was subdivided equally into four equal subgroups (n = 5), according to the used finishing and polishing systems. Mold was made to prepare the specimens with a central hole of 4 mm in diameter and 4 mm in thickness. Light curing tip was placed touching the glass slab for 20 s then the cured specimens were removed from the mold and the specimens immediately and immersed in distilled water at 37°C for 24 h. Surface roughness (Ra) was measured using light sectioning vision system. SEM was done to support the results. RESULTS: The results showed that the lowest (Ra) values were recorded by the specimens under Myler strip followed by Polishing Discs treated specimens followed by Diacomp Plus specimens and the highest (Ra) values were recorded by white polishing stone specimens and regardless to finishing or polishing, it was found that mosaic resin composites group recorded statistically significant (p < 0.05) lower roughness mean value than Harmonize resin composites group. p ≤ 0.05 is statistically significant in all tests. CONCLUSION: Achieving long-lasting esthetics in resin composites restorations needs special attention for obtaining optimal resin polymerization and a perfect surface finish using the appropriate finishing and polishing system.