Warming climates are facilitating the range expansion of many taxa to habitats that were formerly thermally inhospitable, including to higher latitudes and elevations. The potential for such colonization, however, varies widely among taxa. Because environmental factors may interact to affect colonization potential, an understanding of underlying physiological and behavioral mechanisms is necessary to predict how species will respond to potentially suitable habitats. For example, temperature and oxygen availability will interact to shape physiological and performance traits. Our model species, the wall lizard, Podarcis muralis, is a widely distributed ectotherm that continues to expand its range in Europe despite being limited by cold temperatures at high elevations and latitudes. To test the potential for organisms to expand to warming high-altitude environments, we conducted a transplant experiment to quantify the within-individual effects of highaltitude hypoxia on physiological and performance traits. Transplanted lizards maintained individual differences in physiological traits related to oxygen capacity and metabolism (hemoglobin concentration, hematocrit, and peak postexhaustion metabolic rate), as well as performance traits tied to fitness (sprint speed and running endurance). Although lizards altered blood biochemistry to increase oxygen-carrying capacity, their performance was reduced at high altitude. Furthermore, lizards at high altitude suffered a rapid loss of body condition over the 6-wk experiment, suggesting an energetic cost to hypoxia. Taken together, this demonstrates a limited potential for within-individual plasticity to facilitate colonization of novel high-altitude environments.

The insulin and insulin-like signaling (IIS) network is an important mediator of cellular growth and metabolism in animals, and is sensitive to environmental conditions such as temperature and resource availability. The two main hormones of the IIS network, insulin-like growth factor 1 (IGF1) and insulin-like growth factor 2 (IGF2), are present in all vertebrates, yet little is known regarding the responsiveness of IGF2 in particular to external stimuli in non-mammalian animals. We manipulated diet (low quantity of food or high quantity of food) in adult green anole (Anolis carolinensis) females to test the effect of energetic state on hepatic gene expression of IGF1 and IGF2. The absolute expression of IGF2 in female green anoles is 100X higher than IGF1 regardless of diet treatment, and IGF1 and IGF2 expression interact with post-treatment body mass and treatment, as do the purported housekeeping genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and eukaryotic elongation factor 2 (EEF2). The Low Diet group showed a negative relationship between body mass and gene expression for all genes, whereas the relationships between body mass and gene expression in the High Diet group were either absent (in the case of IGF1) or positive (for all other genes). After accounting for total change in mass the Low Diet group expressed IGF2, GAPDH and EEF2 at higher levels compared to individuals in the High Diet group of similar ▵ mass. These results illustrate that expression of IGF1 and IGF2, and housekeeping genes are affected by energe­tic status in reptiles.

Early life adverse conditions can have major consequences on an individual’s life history traits. Oxidative stress has been hypothesized to be one main mechanism underlying the negative consequences of early life adverse conditions. To test this hypothesis, we restricted the food availability of Seba’s short-tailed bat (Carollia perspicillata) mothers of unweaned pups for 10 days, followed by ad libitum provisioning. We also had a control, unrestricted group. We explored the morphological consequences of dietary restriction during early life by measuring growth rate. We also measured four markers of blood oxidative balance during growth. We assessed the level of cortisol, and its inactive form cortisone, in the hair of the pups at the end of growth. Finally, we monitored survival during the first year. Food restriction triggered a slowdown in growth followed by catch-up growth when ad libitum feeding was restored which did not lead to full compensation in size or mass compared to control individuals. We found that higher growth rate was associated with elevated oxidative damage, suggesting an oxidative cost to growth. However, we found no clear evidence for physiological costs specific to the catch-up growth. Survival after a year was not impacted by the treatment, the oxidative balance or the level of glucocorticoids at the end of growth. In conclusion, our results show that individuals were able to efficiently mitigate the short-term consequences of adverse early life conditions. However, consequences might arise in the long-term, and could impact reproductive success or lifespan.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.