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aims to assess the fungicide resistance status of P. viticola for developing a future guideline for downy mildew management of grapes in India.One hundred and sixty downy mildew infected grape leaf samples were collected from 16 vineyards located in Maharashtra, India during September 2015 to November 2016. Most of these vineyards had heavy incidence of downy mildew disease, even though several fungicide interventions for its control were made. The fungicide sensitivity was assessed against kresoxim methyl, dimethomorph, Cymoxanil and Metaxyl-M using leaf disc bioassay, and QoI and CAA resistance gene was detected using allele specific multiplex PCR.Based on the sensitivity response in leaf disc bio-assay, isolates of P. viticola were categorized as, sensitive (S), intermediate (I) or highly resistant (R) to four fungicides. The percentage of sensitive P.

Five mycoparasitic fungi were isolated from sporangiophores of Plasmopara viticola collected from vineyards of five grape growing regions in India. Four isolates were obtained from the P. viticola growth on leaf (M1, M2, M10, and M12_1) and one from growth on berry (M12_2). Morphological observations showed that all isolates belonged to the genus Fusarium.