<p class="PaperAbstract">Cu_xO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO₄, NiSO₄ and H₂SO₄ for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO₄ and NiSO₄ was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process.<strong> </strong>Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled Cu_xO-NiO film. The electrocatalytic behavior of the novel Cu_xO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 µM (S/N = 3) and high sensitivity (1.38 mA mM^-1 cm^-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared Cu_xO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.</p>

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In this work, a low cost glucose and methanol nonenzymatic sensor was prepared using nickel oxide (NiO) nanofilm electrodeposited on a bare Cu electrode. Electrochemical deposition was assisted with cetyl trimethylammonium bromide (CTAB) as a template. Scanning electron microscopy (SEM) was applied to observe the surface morphology of the modified electrode. Cyclic voltammetry (CV) and amperometry techniques were used to study the electrocatalytic behavior of NiO porous film in glucose and methanol detection. For glucose sensing, the electrode showed a linear relationship in the concentration range of 0.01-2.14 mM with a low limit of detection (LOD) 1.7 µM (signal/noise ratio (S/N)=3). Moreover, high sensitivities of 4.02 mA mM −1 cm −2 and 0.38 mA mM −1 cm −2 respectively in glucose and methanol monitoring suggested the modified electrode as an excellent sensor. The NiO-Cu modified electrode was relatively insensitive to common biological interferers. This sensor possessed good poison resistance towards chloride ions, and long term stability and significant selectivity towards glucose and methanol. Finally the proposed sensor was successfully applied for determination of glucose in human blood serum samples.

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