Clathrin, a cytosolic protein composed of heavy and light chain subunits, assembles into a vesicle coat, controlling receptor-mediated endocytosis. To establish clathrin light chain (CLC) function in vivo, we engineered mice lacking CLCa, the major CLC isoform in B lymphocytes, generating animals with CLC-deficient B cells. In CLCa-null mice, the germinal centers have fewer B cells, and they are enriched for IgA-producing cells. This enhanced switch to IgA production in the absence of CLCa was attributable to increased transforming growth factor β receptor 2 (TGFβR2) signaling resulting from defective endocytosis. Internalization of C-X-C chemokine receptor 4 (CXCR4), but not CXCR5, was affected in CLCa-null B cells, and CLC depletion from cell lines affected endocytosis of the δ-opioid receptor, but not the β2-adrenergic receptor, defining a role for CLCs in the uptake of a subset of signaling receptors. This instance of clathrin subunit deletion in vertebrates demonstrates that CLCs contribute to clathrin’s role in vivo by influencing cargo selectivity, a function previously assigned exclusively to adaptor molecules.

The soil mediated allelopathic effect of Echinochloa colona (L.) Link (jungle rice) was investigated on seed germination and seedling growth of Zea mays L. (maize). The experiment was conducted in the Agronomy farm of the University of Agriculture, Faisalabad, Pakistan. This study investigated the effect of different concentrations (1, 2, 3, 4 and 5% on w/w basis) of soil incorporated E. colona plant residues on emergence and seedling growth of maize and compared them with residue free soil (control). The results revealed the significant increase with soil incorporated E. colona residues in maize seedling growth till 2% E. colona plant residues, and an inhibitory effect was noticed over 2% E. colona plant residues in comparison to the control treatment. There was maximum reduction in emergence percentage, root and shoot lengths, root and shoot dry weights and seedling vigor index of maize seedlings at 5% E. colona plant residues. Quercetin, ferulic acid, sinapic acid, syringic acid, cinnamic acid, m-coumaric acid were identified as water soluble phenolic in water extract of E. colona.

Problem statement: In previous researches, we investigated the security of communication channels, which utilizes authentication, key distribution between two parties, error corrections and cost establishment. In the present work, we studied new concepts of Quantum Authentication (QA) and sharing key according to previous points. Approach: This study presented a new protocol concept that allows the session and key generation on-site by independently applying a cascade of two hash functions on a random string of bits at the sender and receiver sides. This protocol however, required a reliable method of authentication. It employed an out-of-band authentication methodology based on quantum theory, which uses entangled pairs of photons. Results: The proposed quantum-authenticated channel is secure in the presence of eavesdropper who has access to both the classical and the quantum channels. Conclusion/Recommendations: The key distribution process using cascaded hash functions provides better security. The concepts presented by this protocol represent a valid approach to the communication security problem

A genetic algorithm is implemented to represent the different shapes of Photonic Crystal Fiber (PCF) profile; this is due to the fact that such fibers have special structure. A novel approach is suggested which differs from the usual fibers. By applying this new approach, more realistic representations of their profiles are drawn and hence highly accurate results are obtained including propagation and dispersion characteristics.