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With the increasing environmental pollution, it is urgently needed to develop efficient methods to remove recalcitrant organic pollutants. Herein, intimately coupled photocatalysis and biodegradation (ICPB) technology was fabricated using bagasse cellulose-TiO2 composite (SBC-TiO2) as the carrier for photocatalyst and biofilm, Trichoderma mixed with activated sludge as a novel biological source for the removal of 1,2,3-Trichlorobenzene (1,2,3-TCB) and 1,3,5-Trichlorobenzene (1,3,5-TCB). The effects of different degradation conditions (carrier dosage, pH of reaction solution, and light intensity) on the degradation efficiency were investigated separately. Under the optimal process conditions (carrier dosage of 5%, pH of the reaction solution was 5, and light intensity was 700 lm/m2), the removal rates of 1,2,3- and 1,3,5-TCB reached 92.02% and 95.00% within 6 h, respectively. The degradation rate of TCB and the variation of microbial dehydrogenase activity (DHA) showed that the ICPB system exhibited good photocatalytic performance and biodegradation stability.