Copper sulfide (CuS) nanoparticles have attracted increasing attention from biomedical ‎researchers across the globe, because of their intriguing properties, which have been ‎mainly explored for energy and catalysis related applications. The aim of the study is to ‎prepare CuS NPs by BSA entrapment to reduce the toxicity, characterizing, comparative ‎the toxicity before and after entrapment against bacteria and check the toxicity against RD ‎and L20B cell lines. CuS-BSA NPs was an easy, low toxicity and low cost chemically ‎synthesized. The CuS-BSA NPs was identified though UV-VIS ‎spectrophotometer, FTIR, XRD, SEM, EDX, and Zeta potential. The antibacterial activity ‎against different G-positive and G-negative bacterial strains have been investigated for (2 ‎mg/ml) concentrations of CuS-BSA NPs and commercial CuS. A result showed that CuS-‎BSA NPs have more antibacterial activity than commercial CuS. Using different ‎parameters of CuS-BSA NPs, its anti-cancer bioactivity for every compound synthesized in ‎RD and L20B cell line was explored, and the result proved there was significant toxicity ‎against RD and L20B cell lines.‎