Fifty plaque samples were collected from teeth. Forty five samples were considered to be positive bacterial isolates about (10 4 bacteria/ml) using selective Ms-agar (Mitis-Salivarius agar) medium. Thirty isolates were considered to be related to the genus Streptococcus and specially to the mutans streptococci of various group; S. sobrinus (serotype D, G), S. mutans (serotype C, F), S. cricetus (serotype A) and S. rattus (serotype B) with percentages of (39.29%), (30.30%), (18.18%) and (3.03%), respectively depending on biochemical and Lancefield grouping identification systems.

The present study investigates the use of the agar dilution method and the inhibitory effects of different concentrations (10%, 20% and 30%) of human, camel and cow's milk on thirteen different species of fungi from the genera Aspergillus, Trichophyton, Microsporum, Penicillium and Fusarium. The results show that all the tested concentrations of each of the three milks were capable of inhibiting the growth of the thirteen fungal species, but the greatest inhibitory effect was recorded with the concentration of 30%. Human milk gave the highest growth inhibition rate on all fungal species and the complete growth inhibition (100%) was recorded in respect to Aspergillus fumigatus with a chi-square x2 value of 9.462. For camel and cow milk, inhibition rates of 96% and 92%, with chi-square-x2 values of 8.684 and 9.140, respectively, were recorded. Overall, the inhibitory effects were observed to be concentration-dependent.

Copper sulfide (CuS) nanoparticles have attracted increasing attention from biomedical ‎researchers across the globe, because of their intriguing properties, which have been ‎mainly explored for energy and catalysis related applications. The aim of the study is to ‎prepare CuS NPs by BSA entrapment to reduce the toxicity, characterizing, comparative ‎the toxicity before and after entrapment against bacteria and check the toxicity against RD ‎and L20B cell lines. CuS-BSA NPs was an easy, low toxicity and low cost chemically ‎synthesized. The CuS-BSA NPs was identified though UV-VIS ‎spectrophotometer, FTIR, XRD, SEM, EDX, and Zeta potential. The antibacterial activity ‎against different G-positive and G-negative bacterial strains have been investigated for (2 ‎mg/ml) concentrations of CuS-BSA NPs and commercial CuS. A result showed that CuS-‎BSA NPs have more antibacterial activity than commercial CuS. Using different ‎parameters of CuS-BSA NPs, its anti-cancer bioactivity for every compound synthesized in ‎RD and L20B cell line was explored, and the result proved there was significant toxicity ‎against RD and L20B cell lines.‎