Isolation and Identification of Mutan's Streptococci Bacteria From Human Dental Plaque Samples

Al-Mudallal, Nada H.; Al-Jumaily, Essam F.

doi:10.22401/jnus.11.3.12

Cited by 18 publications

(19 citation statements)

References 16 publications

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“…Microscopic examination reveal gram positive, spherical shape bacteria arrangement in chain or as pairs. The developing isolates were marked on the center of the MSBA with 1mm diameters and give positive result for rapid diagnosis with (10%) TTC and (4%) Mannitol due to the change of colony color from blue to red Figure. Table 1 shows the results of biochemical test of growth isolates which are consistent with [20].…”

Section: Resultssupporting

confidence: 63%

“…Then a test solution of (10%) mannitol and (4%) of 2,3,5-triphenyltetrazolium chloride (TTC) solution were added on the plates. A change in color to a dark pink due to hydrolysis of mannitol to acid by the enzyme mannitol-1phosphate dehydrogenase and a reduction of (TTC) which could taken as an indicator for the presence of S.mutans [10].…”

Section: Rapid Differentiation Of Coloniesmentioning

confidence: 99%

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Bacteriological Study Of Dental Caries Infection In Baquba

Turki

Mubaruk

2019

MJS

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In the central laboratory of a hospital belonging to Diyala Health Governorate, to isolate and diagnoses Streptococcus mutans bacteria form dental caries infections, to detect the ability of isolate to biofilm formation, the sensitivity of isolates to antibiotics, and effect of plant extracts for Green tea and Capsicum annuum on the growth of the bacteria. The results showed that 68 Isolates belonged to Oral Streptococci were identify from 150 samples taken from dental caries infections, 20 isolates (13.3%) of S. mutans bacteria were obtained. The ability of isolate to form of biofilms by (80%), the isolates varied in their sensitivity to antibiotic, Where the percentages of sensitivity for Tetracycline (70%), Erythromycin (65%), Streptomycin (60%), Ciprofloxacin (50%), whereas resistance to antibiotics Amoxicillin, Ampicillin, Cefepime, Optochin (100%). The value of Minimum Inhibitory Concretion (MIC) of Tetracycline ranging from (256 -1024) micrograms \ ml , Erythromycin ranged from (64 -1024) micrograms \ ml. The inhibition zone of chili pepper and Green tea extracts for test isolates were (30)mm and (27) mm respectively, the comparison of inhibitory effect between plant extract and antibiotics the results indicate that the extracts of chili pepper and green tea have higher inhibitory effect than antibiotics against S.mutans Bacteria .This refers to the possibility of benefiting from those extracts in the manufacture of an alternative treatment, to minimizing the risks of resistance of bacterial as well as reduction the side effects that may be result from the use of s antibiotics , in addition, advantages such plants are easy to get and cheaply priced.

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Section: Resultssupporting

confidence: 63%

Section: Rapid Differentiation Of Coloniesmentioning

confidence: 99%

Bacteriological Study Of Dental Caries Infection In Baquba

Turki

Mubaruk

2019

MJS

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“…Dental plaque is an adherent deposit of bacteria and their products, which forms as a white greenish or even yellow film on all tooth surfaces. Dental plaque accumulates naturally at stagnant or retentive sites formed after one to two days with no oral hygiene [1]. Dental caries is the single most prevalent and costly infectious disease worldwide, affecting more than 90% of the population in the US.…”

Section: Introductionmentioning

confidence: 99%

Streptococcus mutans Dental Caries among Patients Attending Debre Berhan Referral Hospital, Ethiopia

Shenkute¹,

Asfaw²

2019

J Bacteriol Parasitol

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Background: Dental caries is an irreversible microbial disease of the calcified tissues of the teeth. Streptococcus mutans is a bacterial resident of the oral cavity and is considered to be the principal etiological agent of dental caries in humans. Therefore, the aim of this study was to determine the prevalence and risk factors associated with Streptococcus mutans dental caries. Methods: A cross sectional study was conducted among patients who attended Debre Berhan referral hospital dental clinic. Patient's demographic and clinical information was collected by using pre-tested questionnaire. Dental plaques from all patients was picked up by forceps and suspended in to phosphate-buffered saline for further Streptococcus mutans identification. Result: From a total of 115 study participants 56 (48.7%) and 59 (51.3%) were males and females respectively. The overall prevalence of Streptococcus mutans was 79 (68.7%) among patients with dental caries. participants 25 (21.7%) had gem bleeding and 47 (40.9%) of the participants had previous tooth decay. Conclusion: In this study, again Streptococcus mutans is the common public health problem among dental caries patients. Drinking soft drinks, oral debris and gingival index were the associated risk factor for dental caries of Streptococcus mutans.

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“…Single colonies from the surface of MS-agar which considered to be positive were selected for further purification by sub-culturing on the surfaces of MS-agar media. Isolates were first identified depending on their gram-staining (grampositive), microscopic examination (cocci arranged in chains) and catalase test (catalase-negative) (Al-Mudallal et al, 2008). S. mutans isolates enzymatic spectra were tested by the Remel RapID STR system.…”

Section: Isolation Identification and Selection Of The Bacterial Ismentioning

confidence: 99%

Antibacterial effect of green tea and pomegranate peel extracts on Streptococcus mutans of orthodontic treated patients

Abd-El-Aziz

Sallam

2020

Journal of Radiation Research and Applied Sciences

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The antibacterial effect of green tea and pomegranate peel extracts on Streptococcus mutans of orthodontically treated patients and its adherence to the tooth surface were investigated. Streptococcus mutans was isolated from saliva and plaque samples collected from orthodontic treated patients before the start of treatment and 3 months after the treatment. Gamma irradiated (5, and 10 kGy) and un-irradiated extracts of green tea leaves and pomegranate peels were prepared using polar (water, and 70% ethanol) and non-polar (hexane) solvents. The extraction yields, total phenol contents (TPC), and antibacterial activity of all the extracts were investigated. The irradiated (10 kGy) ethanol extracts of both pomegranate peels and green tea contained the maximum amount of TPC and showed superior antibacterial activity (maximum inhibition zone 28, and 35mm) and lowest minimum inhibitory concentration (25.0, and 12.50 mg/ml) against S. mutans, respectively when compared with the other extracts. The effect of various combinations of the irradiated (10 kGy) ethanol extracts of pomegranate peels and green tea on the adherence of S. mutans from orthodontic patients was finally evaluated.

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Isolation and Identification of Mutan's Streptococci Bacteria From Human Dental Plaque Samples

Cited by 18 publications

References 16 publications

Bacteriological Study Of Dental Caries Infection In Baquba

Bacteriological Study Of Dental Caries Infection In Baquba

Streptococcus mutans Dental Caries among Patients Attending Debre Berhan Referral Hospital, Ethiopia

Antibacterial effect of green tea and pomegranate peel extracts on Streptococcus mutans of orthodontic treated patients

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