Choroidal neovascularization (CNV) is the leading cause of vision loss in many blinding diseases, but current antiangiogenic therapies with invasively intravitreal injection suffer from poor patient compliance and a rate of devastating ocular complications. Here, we develop an alternative antiangiogenic agent based on hybrid cell-membrane-cloaked nanoparticles for noninvasively targeted treatment of CNV. The retinal endotheliocyte membrane coating provides as-fabricated nanoagents with homotypic targeting capability and binding ability to the vascular endothelial growth factor. The fusion of red blood cell membranes protects the hybrid membrane-coated nanoparticles from phagocytosis by macrophages. In a laser-induced wet age-related macular degeneration mouse model, a significantly enhanced accumulation is observed in CNV regions after intravenous delivery of the hybrid membrane-coated nanoparticles. Moreover, an excellent therapeutic efficacy is achieved in reducing the leakage and area of CNV. Overall, the biomimetic antiangiogenic nanoagents provide an effective approach for noninvasive treatment of CNV.

Probes featuring room-temperature phosphorescence (RTP) are promising tools for time-resolved imaging. It is worth noting that the time scale of timeresolved bioimaging generally ranges around the microsecond level, because of the short-lived emission. Herein, the first example of millisecond-range time-resolved bioimaging is illustrated, which is enabled through a kind of ultralong aqueous phosphorescence probes (i.e., cyclo-(Arg-Gly-AspD-Tyr-Cys)-conjugated zinc-doped silica nanospheres), with a RTP emission lasting for � 5 s and a lifetime as long as 743.7 ms. We demonstrate that live cells and deep tumor tissue in mice can be specifically targeted through immune-phosphorescence imaging, with a high signal-to-background ratio (SBR) value of � 69 for in vitro imaging, and � 627 for in vivo imaging, respectively. We further show that, compared to that of fluorescence imaging, the SBR enhancement of millisecond-range time-resolved in vivo bioimaging is up to 105 times.

Fluorescence and phosphorescence are knowna s two kinds of fundamental optical signals,which have been used for myriad applications.T od ate,s imultaneous activation of stable fluorescence and long-lived room-temperature phosphorescence (RTP) emission in the aqueous phase remains ab ig challenge.W ep repare zinc-doped silica nanospheres (Zn@SiNSs) with fluorescence and RTPp roperties using af acile hydrothermal synthetic strategy.F or the as-prepared Zn@SiNSs,the recombination of electrons and holes in defects and defect-stabilized excitons derived from oxygen vacancy/ C = Nb onds lead to the production of stable fluorescence and long-lived RTP( emission lasting for % 9s ,q uantum yield (QY): % 33.6 %, RTPl ifetime: % 236 ms). The internal SiÀO bonded networks and hydrophilic surface in Zn@SiNSs can reduce nonradiative decayt of orm self-protective RTP,a nd also provideh igh water solubility,e xcellent pH-and photostability.

Probes featuring room-temperature phosphorescence (RTP) are promising tools for time-resolved imaging. It is worth noting that the time scale of timeresolved bioimaging generally ranges around the microsecond level, because of the short-lived emission. Herein, the first example of millisecond-range time-resolved bioimaging is illustrated, which is enabled through a kind of ultralong aqueous phosphorescence probes (i.e., cyclo-(Arg-Gly-AspD-Tyr-Cys)-conjugated zinc-doped silica nanospheres), with a RTP emission lasting for � 5 s and a lifetime as long as 743.7 ms. We demonstrate that live cells and deep tumor tissue in mice can be specifically targeted through immune-phosphorescence imaging, with a high signal-to-background ratio (SBR) value of � 69 for in vitro imaging, and � 627 for in vivo imaging, respectively. We further show that, compared to that of fluorescence imaging, the SBR enhancement of millisecond-range time-resolved in vivo bioimaging is up to 105 times.

Fluorescence and phosphorescence are knowna s two kinds of fundamental optical signals,which have been used for myriad applications.T od ate,s imultaneous activation of stable fluorescence and long-lived room-temperature phosphorescence (RTP) emission in the aqueous phase remains ab ig challenge.W ep repare zinc-doped silica nanospheres (Zn@SiNSs) with fluorescence and RTPp roperties using af acile hydrothermal synthetic strategy.F or the as-prepared Zn@SiNSs,the recombination of electrons and holes in defects and defect-stabilized excitons derived from oxygen vacancy/ C = Nb onds lead to the production of stable fluorescence and long-lived RTP( emission lasting for % 9s ,q uantum yield (QY): % 33.6 %, RTPl ifetime: % 236 ms). The internal SiÀO bonded networks and hydrophilic surface in Zn@SiNSs can reduce nonradiative decayt of orm self-protective RTP,a nd also provideh igh water solubility,e xcellent pH-and photostability.