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The effect of magnetic field on surface properties of Bacillus cereus CrA and its EPS was studied by zeta potential and FT-IR spectrum. The weak magnetic fields of 6.0-10.0 mT promoted the predominant strain B. cereus CrA to form EPS. The weak magnetic field of 2.4-10.0 mT could improve the bio-adsorption efficiency of anions by decreasing the negative charge of the intact strain, while the magnetic field of 17.4 mT could increase the negative charge of the strain's EPS by 20% and be responsible for the bio-adsorption of cations. Magnetic field could affect the band intensity and the slight shifting of peaks and the fluctuation of the bacterial absorption capability depended on the magnetic density. The magnetic field energy could affect the hydroxyl group and carboxyl group by forming hydrogen bonding, which were responsible for the adsorption ability of the strain and its EPS.