Distribution of tetracycline resistance genes and AmpC β-lactamase genes in representative non-urban sewage plants and correlations with treatment processes and heavy metals

Xu, Yanbin; Hou, Mao-Yu; Li, Yafei; Huang, Lu; Ruan, Jun; Zheng, Li; Qiao, Qi; Du, Qingping

doi:10.1016/j.chemosphere.2016.12.027

Cited by 59 publications

(28 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 There are clearly established links between the resistance genes found in soil and animals and resistance genes found in humans, including human pathogenic bacteria, with antibiotic resistance genes found in the former also present in human pathogens. 11,12 These links have been seen in diverse environments that include industrial, 13,14 clinical, 15 aquatic 13,16 (including wastewater) 17 and, especially, agricultural [18][19][20][21][22] settings.…”

Section: Introductionmentioning

confidence: 99%

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

et al. 2019

View full text Add to dashboard Cite

BACKGROUND Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics. RESULTS Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole. CONCLUSION Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry

show abstract

Section: Introductionmentioning

confidence: 99%

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Sulfonamides are old antibiotics that have been extensively used in the past but are not used anymore for human consumption because of its toxicity. However, it is still used in agriculture, and the genes encoding resistance for these antibiotics were found to be persistent 34,35 . A study found that genes conferring resistance to fluoroquinolones (qnrS, aac(6′)-Ibcr) were more likely to co-occur with ampC in a plasmid of Serratia marcescens 36 .…”

Section: Resultsmentioning

confidence: 99%

Seasonality impels the antibiotic resistance in Kelani River of the emerging economy of Sri Lanka

2020

View full text Add to dashboard Cite

We evaluated the occurrence of antibiotic-resistant bacteria, antibiotic-resistant gene, and metal concentration in a tropical river of Sri Lanka as a pre-emptive effort to understand the seasonal impact on their prevalence. Resistance for norfloxacin, ciprofloxacin, levofloxacin, kanamycin monosulfate (KM), tetracycline (TC), and sulfamethoxazole (ST) was measured with Kirby-Bauer disc diffusion method. The prevalence of Escherichia coli ranged from 10 to 27 CFU (colony-forming unit) ml −1 in Kelani River in Sri Lanka, and most of the E. coli isolates were resistant to more than one antibiotic. However, the resistance for TC and ST was much higher than other antibiotics. We found that the resistance percentage for older antibiotics like TC and ST was higher than the newer antibiotics. We detected genes that confer resistance to TCs, sulfonamides, β-lactams, and fluoroquinolones. Seasonal variation in the resistance of fluoroquinolones was much higher than the non-fluoroquinolones, but the effect was antagonistic. Overall, the significant seasonal variations imply the importance of mixed source and environmental conditions for development and transmission of antibiotic resistance.

show abstract

“…Tet A followed with a relative abundance of 0.013. Probably due to the widespread adoption as feed additives and anti‐infectious antibiotics in livestock waste, sulfonamide and tetracycline resistance genes were frequently described as the two most prevalent ARGs (Heuer & Smalla, ; Xu et al., ; Zhang, Zhang, et al., ). Int I, as an important carrier for ARG transfer, was also prevalent in our results, with a relative abundance of 0.015.…”

Section: Resultsmentioning

confidence: 99%

“…The abundances of all ARGs were clearly reduced after sedimentation treatment, with reduction efficiencies of 0.3–4.0 log. This efficient reduction has been hypothesized to stem from the sedimentation from wastewater to sludge (Xu et al., ). This speculation is supported by our previous study that biosolids had significantly more ARGs’ abundance than the treated effluent of secondary sedimentation (Yuan et al., ).…”

Section: Resultsmentioning

confidence: 99%

“…A few studies describe the positive correlation between ARGs and metals (like Hg, Zn, Pb, and Cu) in the environment (Mao et al, 2015;Zhu et al, 2013). The co-occurrence between ARGs and metal resistance genes (MRGs) is also reported (Xu et al, 2017). Specific microorganism community members are other essential factors that affect the propagation of antibiotic resistance by increasing horizontal gene transfer (Guo, Yuan, & Yang, 2015a;Li, Li, & Zhang, 2015).…”

Section: Research Articlementioning

confidence: 99%

See 1 more Smart Citation

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

Yuan

Zhai

Mao

et al. 2019

Water Environment Research

View full text Add to dashboard Cite

This study explores the prevalence, emission, and reduction of five ARGs (sulI, tetA, mphB, qnrD, and mcr‐1) and integron (intI) through a distributed swine wastewater purification facility and the effluent‐receiving environment. Typical metal resistance genes (MRGs), pathogenic bacterial indicators, the bacterial community, and wastewater properties were also explored to determine their effects on the fates of ARGs. Results indicated that the purification process could hardly effectively remove ARGs’ prevalence. 3.1 × 104–7.1 × 108 copies/L were present after purification, and 4%–57% of them persisted in the subsequent creek and adjacent soil. 16S rRNA sequencing suggested that the discharge of wastewater significantly changed the bacterial community in receiving creek and soil. Molecular ecological networks analysis detected the wide co‐occurrence among ARGs, MRGs, and PBGs, which could further facilitate the propagation of antibiotic resistance. ARG incidence and specific bacterial genera were closely correlated, suggesting an extensive hosting relationship. Redundancy analyses showed wastewater organics and nutrients showed positive correlation to most ARGs’ abundance, but negatively correlated to their relative abundance. Practitioner points Fate of five ARGs and intI was studied in a swine wastewater treatment system. The treatment process could not effectively reduce ARGs’ abundance. ARGs and pathogens in wastewater were transferred to the receiving creek and soil. The network analysis found wide co‐occurrence among ARGs, metal resistance genes, and pathogens. Wastewater nutrients positively correlated to ARG's abundance but negatively correlated to their relative abundance.

show abstract

Distribution of tetracycline resistance genes and AmpC β-lactamase genes in representative non-urban sewage plants and correlations with treatment processes and heavy metals

Cited by 59 publications

References 51 publications

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

Seasonality impels the antibiotic resistance in Kelani River of the emerging economy of Sri Lanka

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

Contact Info

Product

Resources

About