BackgroundIn the bone marrow, hematopietic and mesenchymal stem cells form a unique niche in which the oxygen tension is low. Hypoxia may have a role in maintaining stem cell fate, self renewal and multipotency. However, whereas most studies addressed the effect of transient in vitro exposure of MSC to hypoxia, permanent culture under hypoxia should reflect the better physiological conditions.ResultsMorphologic studies, differentiation and transcriptional profiling experiments were performed on MSC cultured in normoxia (21% O2) versus hypoxia (5% O2) for up to passage 2. Cells at passage 0 and at passage 2 were compared, and those at passage 0 in hypoxia generated fewer and smaller colonies than in normoxia. In parallel, MSC displayed (>4 fold) inhibition of genes involved in DNA metabolism, cell cycle progression and chromosome cohesion whereas transcripts involved in adhesion and metabolism (CD93, ESAM, VWF, PLVAP, ANGPT2, LEP, TCF1) were stimulated. Compared to normoxic cells, hypoxic cells were morphologically undifferentiated and contained less mitochondrias. After this lag phase, cells at passage 2 in hypoxia outgrew the cells cultured in normoxia and displayed an enhanced expression of genes (4-60 fold) involved in extracellular matrix assembly (SMOC2), neural and muscle development (NOG, GPR56, SNTG2, LAMA) and epithelial development (DMKN). This group described herein for the first time was assigned by the Gene Ontology program to "plasticity".ConclusionThe duration of hypoxemia is a critical parameter in the differentiation capacity of MSC. Even in growth promoting conditions, hypoxia enhanced a genetic program that maintained the cells undifferentiated and multipotent. This condition may better reflect the in vivo gene signature of MSC, with potential implications in regenerative medicine.

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Rays are among the largest fishes and evolved from shark-like ancestors nearly 200 million years ago. They share with sharks many life history traits: all species are carnivores or scavengers; all reproduce by internal fertilisation; and all have similar morphological and anatomical characteristics, such as skeletons built of cartilage. Rays of the World is the first complete pictorial atlas of the world’s ray fauna and includes information on many species only recently discovered by scientists while undertaking research for the book. It includes all 26 families and 633 valid named species of rays, but additional undescribed species exist for many groups. Rays of the World features a unique collection of paintings of all living species by Australian natural history artist Lindsay Marshall, compiled as part of a multinational research initiative, the Chondrichthyan Tree of Life Project. Images sourced from around the planet were used by the artist to illustrate the fauna. This comprehensive overview of the world’s ray fauna summarises information such as general identifying features and distributional information about these iconic, but surprisingly poorly known, fishes. It will enable readers to gain a better understanding of the rich diversity of rays and promote wider public interest in the group. Rays of the World is an ideal reference for a wide range of readers, including conservationists, fishery managers, scientists, fishers, divers, students and book collectors.