No abstract

Lower limb IPC protects against systemic inflammation and acute lung injury in lower limb I/R injury.

Background: Interleukin (IL) 10 is a potent anti-inflammatory cytokine. Disruption of the IL-I0 gene in C57/Black6 rnice results in enterocolitis in the presence of intestinal bacteria. This study investigated gut mucosal barrier function sequentially during the development of colitis in this modelo Methods: Animals were bred in specific pathogen-free conditions and transferred to convencional housing at 4weeks. Mice were evaluated at 6,8,10,12,14 and 15weeks ofage. Barrler function was assessed by measuring intestinal permeability and antibody response to systernic endotoxaernia (antibody to the core glycolipid region of lipopolysaccharide; EndoCAb). Colons were harvested and a histological injury score (IllS) was calculated. Results: The ms increased progressively until 12 weeks, with an associated increase in intestinal permeability, and irnmunoglobulin (Ig) M and IgG EndoCAb. The ms correlated positively with both intestinal permeability and IgM and IgG EndoCAb. Intestinal permeability showed a positive correlation with EndoCAb. Conclusion: n.-IO knockout rnice develop coli*1h an associated disturbance in gut mucosal barrier function, as measured by increased ~ermeability and endotoxaernia. The colitis found in the IL-I0 knockout mouse

Serious complications are common after elective open AAA repair, and we have shown for the first time that a restricted perioperative fluid regimen can prevent MC and significantly reduce overall hospital stay.

This study evaluates the effect of experimental biliary obstruction by bile duct ligation (BDL) and biliary drainage on cell-mediated immunity in Wistar rats. Immune status has been assessed by a mitogen stimulation test of T lymphocytes with phytohaemagglutinin. Animals were followed for up to 35 days after BDL. Regression analysis showed a significant negative correlation between lymphocyte function and the period of jaundice (correlation coefficient -0.57, P less than 0.001). Following BDL for 21 days, groups of animals had internal biliary drainage for 7, 14 and 28 days, and external drainage for 14 days. Compared with obstructed animals, 14 days internal drainage was required to improve lymphocyte function (P less than 0.05). Animals which had 14 days of external drainage had significantly lower lymphocyte stimulation than internal drainage animals (P less than 0.05). The results demonstrate that obstructive jaundice produces a progressive reduction of T lymphocyte function. This can be reversed by biliary drainage, internal drainage being more effective than external drainage.