This investigation aims to determine the effect of the same polishing protocol on the surface roughness (Ra) of different resins obtained by different processing techniques. Acrylic resins obtained by CAD/CAM technology overcame the disadvantages identified in conventional materials. A total of thirty samples (six of each resin): self-cured, heat-polymerized, injection molded, CAD/CAM 3D-printed and CAD/CAM milled were prepared. JOTA® Kit 1877 DENTUR POLISH was used to polish the samples by two techniques: manual and mechanized, with a prototype for guided polishing exclusively developed for this investigation. The Ra was measured by a profilometer. The values were analyzed using ANOVA, Games–Howell post-hoc test and One-sample t-test, with p < 0.05. Manual polishing produces lower values of Ra compared to mechanized polishing, except for injected molded resins (p = 0.713). Manual polishing reveals significant differences between the resin pairs milling/3D-printing (p = 0.012) and thermopolymerizable/milling (p = 0.024). In the mechanized technique only, significant differences regarding the Ra values were found between the self-cured/3D-printed (p = 0.004) and self-cured/thermopolymerizable pair resins (p = 0.004). Differences in surface roughness values can be attributed to the inherent characteristics of the resin and the respective processing techniques.
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