We studied the polyphenol composition and antioxidant activity of the ethanolic and ethyl acetate extracts of Hypericum connatum (Guttiferae), in addition to their antimicrobial and antiquorum-sensing (AQS) properties. The extracts exhibited a similar polyphenol content and a notable antioxidant activity, which was stronger in the ethanolic extract (EC50=3.2 μg/mL). Ultrahigh-performance liquid chromatography analyses revealed different polyphenol profiles, with rutin and apigen being the main components of the ethanolic extract and caffeic acid, (-)-epicatechin and p-coumaric acid being abundant in the ethyl acetate extract. The ethyl acetate extract exhibited a more effective antimicrobial activity than the ethanolic extract against the pathogens Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa. For the first time, AQS activity was detected in H. connatum: the ethanolic extract inhibited the production of violacein, a quorum-sensing-regulated pigment, in a Chromobacterium violaceum tester strain, without interfering with its growth.
De Feo (2016) The protective effect of Hypericumconnatum on stress-induced escape deficit in rat is related to its flavonoid content, Pharmaceutical Biology, 54:9, 1782-1792, DOI: 10.3109/13880209.2015 Objective This study evaluates the antidepressant-like effect of H. connatum in rats and identifies the components involved in this activity. Materials and methods First, the effects of acute and 14-d oral administrations of an extract derived from H. connatum aerial parts were studied using the Escape Deficit (ED) test. Next, methanol-extracted flavonoid-enriched fractions B and C and fraction-purified flavonoids (quercetin, rutin and isoquercitrin) were evaluated in the ED test after acute administration. To rule out possible confounding effects of the flavonoids, we examined nociceptive threshold using the tailflick test and anxious behaviour using the elevated plus maze (EPM) test. Results Hypericum connatum increased reactivity of unavoidable stress-exposed rats after acute
We evaluated fruit availability, phytochemical characteristics and mineral content of R. rubiginosa L. rose hips, at two experimental sites in San Luis province: Potrero de los Funes-El Volcán (PF-V) and Estancia Grande (EG). Plant density was 70 plants/ha (1 ha = 10,000 m 2 ) in PF-V and 50 plants/ha in EG. Fruit availability (D F ) in fresh fruits (FF) was 4.39 kg (PF-V) and 3.10 kg (EG) during March, and decreased during June. Ascorbic acid in FF increased during ripening period, peaking on late April, early May, and diminished 75% -85% in dried fruits. Total soluble solids in FF ranged from 32.55 to 55.20 ˚Brix. Elements Ba, Ca, Fe, K, Zn and Cu, determined by inductively coupled plasma optical emission spectrometry (ICP-OES), were significant for human health. Dried fruits maintain a high level of ascorbic acid, a characteristic added to content of mineral elements, particularly calcium content, providing an important nutritional value to R. rubiginosa rose hips.
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