Leptospirosis is widespread throughout the world. In the bovine species, economic losses are mainly associated with reproductive failures. Due to its importance, the objective of the work was to determine the serovarieties most related to bovine leptospirosis in southwest Goiás, Brazil. 2,006 samples were collected in two slaughterhouses in 14 of the 18 municipalities in that region. For the diagnosis, the blood serum samples were submitted to the Microscopic Soroagglutination test (SAM), against 32 serovarieties of leptospires, adopting a positive titer greater than 100. A geographical distribution of the positive animals and the main serovarities present in the southwest of Goiás was carried out, and also a questionnaire with the producers, containing epidemiological information, the vaccine being one of the prophylactic measures related to the infection, on the other hand it was also observed that vaccinated animals they have also been shown to be reactive. It was noted 1,406 (70.1%) cattle were seroreactive for at least one of the 32 serovarieties of Lepstospira ssp. tested. The most frequent serovarieties were Guaricura (30.80%), Wolffi (30.51%), Shermani (9.39%), Hardjo (7.54%), Bananal (4.27%) and Pomona (3.27) %). Serranópolis (77.9%), Perolândia (75.5%), Caiapônia (74.7%) and Jataí (73.2%) are the municipalities with the highest frequency of seroreagent animals. Through this research, a high index of seroreactive animals from the studied region was demonstrated, reinforcing the importance of leptospirosis infection in the Southwest region of the State, being necessary, therefore, the implementation of general measures of control and infection of the animals.

A brucelose bovina é uma enfermidade infecciosa, crônica e de notificação obrigatória, responsável por inúmeras perdas econômicas e estabelecimento de barreiras sanitárias. O estudo foi realizado com o intuito de analisar a tendência de longo prazo das taxas de vacinação na microrregião do Sudoeste Goiano, no período de 2015 a 2020. Calculou-se as taxas de vacinação contra a brucelose bovina para, posteriormente, realizar os cálculos de tendência temporal de vacinação e suas análises. Os dados de vacinação do rebanho bovino, do período em questão, foram fornecidos pela Agência Goiana de Defesa Agropecuária (Agrodefesa), Goiânia-Go. No Sudoeste Goiano, a taxa de vacinação contra a brucelose foi de 59% no primeiro semestre de 2015, e de 35% no segundo semestre do mesmo ano. Em 2017, no primeiro semestre, a taxa foi de 54%, e de 29% no segundo semestre. No primeiro semestre de 2018, a taxa de vacinação observada foi de 48%, e de 25% no segundo semestre. No primeiro e segundo semestres de 2019, foram observadas, respectivamente, taxas de vacinação de 58% e 29%. Por fim, no primeiro semestre de 2020, observou-se uma taxa de 58%. A média das taxas de vacinação nos semestres avaliados foi de 44%. A menor taxa de vacinação foi observada em Chapadão do Céu (8%) no segundo semestre de 2017, com taxa de variação semestral de – 15,13% (IC95% - 28,7 a 1). Santa Rita do Araguaia apresentou as duas maiores taxas de vacinação, a primeira, 117%, observada no primeiro semestre de 2020, com taxa de variação semestral de - 8,57% (IC95% - 20,2 a 4,8), e a segunda, 92%, observada no primeiro semestre de 2017, com taxa de variação semestral de – 8,57% (IC95% - 20,2 a 4,8). As tendências de longo prazo das taxas de vacinação foram de estabilidade na maioria dos municípios avaliados. Uma vez que grande parte das taxas foram inferiores a 70% em todos os anos avaliados, essa estabilidade se torna desfavorável. Os resultados nos sugerem pouca evolução no programa de vacinação contra a brucelose bovina no Sudoeste Goiano o que pode influenciar negativamente a prevalência da doença na região.

The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent assay (I-ELISA) and the competitive enzyme-linked immunosorbent assay (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. The V1 of I-ELISA identifies them through the monoclonal (M23) anti-bovine IgG (I-ELISAM23) and the V2 through the ProteinA / G (I-ELISA-A/G). Serum samples of 862 buffaloes (Bubalus bubalis) from the Northeast of Argentina (NEA) were analyzed using the complement fixation test (CFT) as the reference. Receiving Operator Characteristic (ROC) analysis defined for the area under the curve (AUC) determined the cutoff points, sensitivity (Se) and specificity (Sp) for each test. CFT identified 107 positive and 755 negative sera. The best AUC (0.986), Concordance with CFT (96.3%) and kappa value (0.843) was obtained by I-ELISA A/G test. This assay showed the highest Se (95.33%) and C-ELISA the highest Sp (97%). FPA failed to measure the antibodies in 23 (2.65%) serum samples due to unsuccessful reading. I-ELISA M23 proved to be ineffective to diagnose brucellosis in bubaline sera. The four serological tests showed cutoff points lower than those standardized for bovines. As conclusion, I-ELISA A/G, C-ELISA and FPA with its limitations would be effective techniques for the diagnosis of brucellosis in buffaloes in the NEA, requiring an appropriate cut-off point to guarantee their maximum performance in this species.