Atualmente, os alimentos prontos para consumo têm se popularizado. Isto se deve ao fato da conveniência e propriedades nutricionais encontradas nesses produtos. Embora sofram processamento tecnológico na fabricação, falhas higiênico-sanitárias podem se tornar um sério risco à saúde do consumidor devido à veiculação de patógenos. Dentre estes, destaca-se o gênero Listeria que tem importância para a indústria de alimentos e saúde pública. Assim, objetivou-se neste estudo a detecção de Listeria spp. em salames tipo Italiano fatiados, embalados a vácuo e comercializados em supermercados do bairro de Casa Amarela, Recife-PE. Foram coletadas 22 amostras de salames tipo Italiano fatiados, embalados a vácuo das três principais marcas brasileiras, estas foram analisadas de acordo com a metodologia da Instrução Normativa de nº62/2003 do Ministério da Agricultura Pecuária e Abastecimento.  Dentre as amostras estudadas foi detectada uma ocorrência de 13,64% (3/22), este dado revela possíveis falhas na elaboração do alimento estudado, além de evidenciar a presença de Listeria spp. em salames tipo Italiano fatiados e embalados a vácuo.

Investigation of Listeria spp. and Salmonella spp. in curd cheese produced and distributed in the county of Venturosa-Pernambuco, Brazil [Pesquisa de Listeria spp. e Salmonella spp. em queijo coalho produzido e comercializado no município de Venturosa-Pernambuco, Brasil] "Artigo Científico/Scientific Article"

El objetivo de este estudio fue evaluar la producción de biofilm, el perfil de susceptibilidad y la detección de genes de resistencia presentes en aislados de Salmonella spp de canales frescas de pollo vendidas en una metrópoli brasileña. De un total de 61 muestras de canales frescas de aves de corral, 21 fueron positivas para la presencia de Salmonella spp. En cuanto a la prueba de sensibilidad antimicrobiana, (13/21) los aislamientos probados fueron resistentes a al menos un antibiótico, correspondiente al 61,9%, y el 38% (08/21) fueron resistentes a múltiples fármacos. Se identificaron al menos dos genes de resistencia en todos los aislamientos, especialmente los genes relacionados con la resistencia a β-lactamasas y quinolonas. También se observó que algunos aislados de Salmonella spp mostraron patrones genéticos idénticos. Y los 21 aislados fueron capaces de formar biopelícula. La identificación del biofilm de Salmonella spp. formando y portando diferentes genes de β-lactamasa y determinantes de resistencia a quinolonas demuestra la capacidad de estas bacterias para acumular diversos mecanismos de virulencia y resistencia a antimicrobianos. Por lo tanto, la propagación de diferentes grupos clonales de Salmonella spp. MDR en canales de carne de aves de corral expresada en este atestiguan la necesidad de controles efectivos para contener este microorganismo, que además de ser un riesgo para la salud pública, también es responsable de considerables pérdidas económicas.

Background: Urinary tract infection in dogs is usually associated with the presence of bacteria, with a higher prevalence of Gram-negative bacteria, represented mainly by enteric bacteria such as Escherichia coli and Proteus spp., followed by Gram-positive bacteria such as Staphylococcus spp., and Streptococcus spp. There are scant reports of Salmonella spp. as the causative agent of urinary tract infection in dogs. Indeed, the literature describes only a few cases, most of which involve the isolation of these bacteria in feces. This paper reports a case of canine cystitis caused by Salmonella enterica subsp. enterica in the northeast region of Brazil. Case: A female dog of the Fila Brasileiro breed, about 9 year-old, wormed but unvaccinated, was evaluated at the Veterinary Hospital of the Federal University of Pernambuco -UFRPE. The dog showed clinical signs of apathy, cachexia, polyphagia, polyuria and opacity of the crystalline lens. The dog's owner stated that the animal was fed with commercial dog food. In the clinical exam, the patient presented pale mucosa, cachexia, absence of ectoparasites, and her rectal temperature was 39.5°C. Moreover, cardiorespiratory auscultation of the patient revealed tachycardia (190 bpm) and tachypnea (36 bpm). The owner's main complaint was the clinical condition of frequent urination (polyuria). A urinalysis and urine culture with antibiogram were requested as complementary exams, after collecting the urine by cystocentesis. The volume obtained in the physical examination of urinalysis was 7 mL of yellow urine with a putrid smell, cloudy appearance and density of 1.024. The chemical examination revealed pH 6.5, protein (+++), bilirubin (+), normal urobilinogen and negative reactions for glycoses, ketone, nitrite and urine occult blood. Bacteriuria and pyuria were detected in a urine sediment test. Urine was cultured on blood agar and Levine agar in a bacteriological incubator at 37°C under aerobiosis, for 24 h. This culture produced an exuberant and pure growth of glossy grey bacterial colonies on blood agar and glossy colonies on Levine agar. The Gram test revealed gram-negative bacilli. The sample was subjected to biochemical tests to identify Gram-negative enterobacteria, whose results provided a presumptive identification of Salmonella species. The microbial species was identified using a VITEK 2 Compact ® , and was followed by a serology test for the identification of the serogroup using a polyvalent serum, which enabled the identification of Salmonella enterica subsp. enterica. The antibiogram showed sensitivity to ciprofloxacin, gentamicin and penicillin, and resistance to amoxicillin and ampicillin. Discussion: Clinical signs of cachexia and polyuria may be related to canine urinary tract infection caused by Salmonella enterica subsp. enterica, since these symptoms had already been recorded previously in a case of a bacterial infection by the same serogroup. Isolation of Salmonella spp. in a non-selective medium was determinant in identifying these bacteria. Since the...

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