No abstract

Gilthead seabream, Sparus aurata L., fed diets of equal vitamin C content were exposed to three different stressors-hyposalinity, shallow water and handlingand the effect of each on the internal homeostasis of the fish was evaluated. The individuals subjected to hyposaline stress exhibited no significant changes in plasma osmolality, Na*, Cl", K+, glucose, protein and triglyceride concentrations. Hepatic glycogen content was significantly lower in the fish subjected to the lower salinity (12%o), while the red muscle glycogen was unaltered. Amongst the challenged individuals, tissue ascorbate content was not significantly altered. Shallow water stress significantly diminished the liver and red muscle glycogen content, and lowered the liver, kidney and spleen vitamin C concentrations in the stressed fish. No significant changes were recorded in the plasma glucose, protein and triglyceride concentrations. In response to handling stress, no significant differences were detected in any of the measured parameters, with the exception of plasma glucose levels and the splenic L-ascorbic acid and ascorbate-2sulphate (vitamin C2) contents. The lack of existing standardization of both experimental designs and analytical methods made comparisons of the present data with the work of other authors difficult, thus highlighting the necessity of coordinated research in this field.

Hypoxia (55% DO) and ascorbic acid (AA) supplementation level (0, 10, 25 and 50 mg AA equivalent kg 1 diet, supplied as ascorbyl polyphosphate) did not significantly alter juvenile sea bream Sparus aurata growth, body composition, survival and skin hydroxyproline concentration. Liver, head kidney and spleen AA concentrations significantly decreased with decreasing dietary AA level, but were not influenced by hypoxia. This study showed that sea bream AA requirements were not influenced by chronic hypoxic stress. 2002 The Fisheries Society of the British Isles

We report a case of a man presenting with an unexplained fever, pancytopenia and hepatosplenomegaly without lymphadenopathy. Bone marrow flow cytometry strongly suggested hepatosplenic γδ T-cell lymphoma and infiltration of bone marrow samples by pathological T-lymphocytes confirmed the diagnosis. Despite chemotherapy the patient died 1 year after diagnosis. This is a rare disease that should be considered in the differential diagnosis of hepatosplenomegaly especially when it presents with B-symptoms and no lymph node enlargement. There is no standard therapy and the prognosis is poor.