In aquaculture, microsatellite DNA markers are used to genotype parental broodstock, to assess fertilization success, and to maintain pedigree information for selective breeding. In this study we genotyped individual Haliotis asinina larvae by analyzing a suit of polymorphic microsatellite loci. At least 10 loci can be analyzed from a single abalone veliger larva. We assayed 5 polymorphic loci to identify the parents of individual larvae produced in 3 separate crosses. In all cases, the parents of an individual veliger could be determined from as few as 3 loci. The microsatellite analysis revealed that, in each of our crosses, a single male fathered most of the veligers, despite efforts to normalize the amount of sperm contributed by competing males. These observations suggest that highly controlled breeding practices may be required to ensure that the genetic diversity of an abalone population produced for aquaculture is maintained at the level of diversity of the original broodstock.

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Issues related to artificial intelligence (AI) and ethics have gained much traction worldwide. The impact of AI on society has been extensively discussed. This study presents a bibliometric analysis of research results, citation relationships among researchers, and highly referenced journals on AI and ethics on a global scale. Papers published on AI and ethics were recovered from the Microsoft Academic Graph Collection data set, and the subject terms included “artificial intelligence” and “ethics.” With 66 nations’ researchers contributing to AI and ethics research, 1585 papers on AI and ethics were recovered, up to 5 July 2021. North America, Western Europe, and East Asia were the regions with the highest productivity. The top ten nations produced about 94.37% of the wide variety of papers. The United States accounted for 47.59% (286 articles) of all papers. Switzerland had the highest research production with a million-person ratio (1.39) when adjusted for populace size. It was followed by the Netherlands (1.26) and the United Kingdom (1.19). The most productive authors were found to be Khatib, O. (n = 10), Verner, I. (n = 9), Bekey, G. A. (n = 7), Gennert, M. A. (n = 7), and Chatila, R., (n = 7). Current research shows that research on artificial intelligence and ethics has evolved dramatically over the past 70 years. Moreover, the United States is more involved with AI and ethics research than developing or emerging countries.