RESUMENObjetivos Investigar la prevalencia y sensibilidad de gérmenes aislados en recién nacidos hospitalizados en un hospital de referencia de Bogotá. Proponer un esquema antibiótico inicial en infecciones neonatales. Métodos Se analizaron los cultivos para aerobios y aerobios facultativos practicados entre febrero y diciembre del 2002. Los hemocultivos se procesaron en BacT/ALERT (Dirham, NC); los urocultivos en UROCULT (BioBacter) y las puntas de catéter en Tioglicolato. La identificación se hizo con BBL CRYSTAL (BD, Sparks, MD). La sensibilidad se determinó por difusión de disco. Resultados Fueron positivos 1 097 de 3 710 cultivos; se aislaron 64,3 % Gram-positivos, 30,6 % Gram-negativos y 4,9 % Candidas. Los Gram-positivos aislados fueron: estafilococos coagulasa negativa (64,2 %); Enterococcus (13,8 %) y estafilococos coagulasa positiva (13,3 %). Los Gram-negativos mas frecuentes fueron Klebsielas (45,2 %); Escherichia coli (30,9 %) y Serratias (10,1 %). El 64 % de los estafilococos coagulasa negativos fueron Stafilococcus epidermidis. La sensibilidad del S. epidermidis y los estafilococos coagulasa positivos a la vancomicina fue del 100 %. Hubo 86,4 % (IC95%: 82,3-89,9) de resistencia de los estafilococos coagulasa negativos a los beta-lactámicos. La sensibilidad de los Gram-negativos fue del 98,1 % REVISTA DE SALUD PÚBLICA • Volumen 7 (2), Julio 2005 192 (IC95%: 89,9-99,9) a imipenem, 78,1 % (IC95%: 64,9-88,2) a gentamicina y 46,6 % (IC95%: 28,3-65,7) a amikacina. Conclusiones Se encontró una alta prevalencia de estafilococos coagulasa negativos particularmente de S. epidermidis. No se observó resistencia de S. epidermidis ni estafilococos coagulasa positivos a vancomicina. Se observa resistencia creciente de los estafilcocos coagulasa negativos a oxacilina y de los Gram-negativos a amikacina Palabras Clave: Stafilococcus epidermidis, infecciones estafilocócicas, recién nacido, infección hospitalaria, tests de sensibilidad microbiana (fuente: DeCS, BIREME). ABSTRACT Microbiological profiling of isolates from the Neonatal Unit of a thirdlevel hospital in Bogotá, ColombiaObjectives Investigating the prevalence and sensitivity of germs isolated from newborn in a referral hospital in Bogotá. Suggesting an empirical antibiotic treatment for neonatal infection. Methods Cultures taken between February and December 2002 were analysed. Blood cultures were processed using BacT/ALERT (Durham, NC), urine cultures by UROCULT (Bio-Bacter) and catheter tips in thioglycollate. BBL CRYSTAL identification system (BD, Sparks, MD) was used for identifying germs. Antibiotic sensitivity was determined by disk diffusion. Results There were 1,097 positive aerobic and facultative aerobic germ cultures; 64,3% were Gram-positive, 30,6% Gram-negative and 4,9% were yeasts. Gram-positive germs consisted of coagulase-negative staphylococci (64,2%), enterococcus (13,8%) and coagulase-positive staphylococci (13,3%). The most frequent Gram-negatives were Klebsiella (45,2 %), Eschericha coli (30,9 %) and Serratia (10,1%). Staphylococc...

Background By March 2022, around 34 million people in Colombia had received a complete scheme of vaccines against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including, mRNA-based vaccines, viral vectored coronavirus vaccines, or the inactivated whole virus vaccine. However, as several SARS CoV 2 variants of concern (VOC) and interest (VOI) cocirculate in the country, determining the resistance level to vaccine elicited neutralizing antibodies (nAbs) is useful to improve the efficacy of COVID-19 vaccination programs. Methods Microneutralization assays with the most prevalent SARS-CoV-2 lineages in Colombia during 2020-2021 were performed using serum samples from immunologically naive individuals between 9 and 13 weeks after receiving complete regimens of CoronaVac, BNT162b2, ChAdOx1, or Ad26.COV2.S. The mean neutralization titer (MN50) was calculated by the Reed-Muench method and used to determine differences in vaccine elicited nAbs against the SARS-CoV-2 lineages B.1.111, P.1 (Gamma), B.1.621 (Mu), and AY.25.1 (Delta). Results The most administered vaccines in the country, BNT162b2 and CoronaVac, elicited significantly different nAb responses against Mu, as the GMTs were 75.7 and 5.9 fold lower relative to the control lineage (B.1.111), while for Delta were 15.8 and 1.1-fold lower, respectively. In contrast, nAb responses against Mu and Delta were comparable between ChAd0x1-s and Ad26.COV2.S as the GMTs remained around 5 to 7 fold lower relative to B.1.111. Conclusions The emergence of SARS-CoV-2 variants in Colombia with a significant capacity to escape from vaccine elicited nAbs indicates that a booster dose is highly recommended. Furthermore, other non-pharmacological measures should be retained in the vaccinated population.