The issues of biological conversion of products of processing plant raw materials remain invariably relevant. The development of processing secondary resources allows to increase the efficiency of food production, solving environmental problems and to contribute to ensuring food security. The paper presents data on the results of studies of biotechnological properties of yeast cultures Zygosaccharomyces kombuchaensis sp. and bacteria Gluconoacetobacter xylinus (Brown 1886) Yamada et al 1998, constituting a symbiotic culture called SCOBY (symbiotic culture of bacreties and yeasts), adapted and cultivated in the Krasnodar Territory with the aim of their further use for biological conversion of fruit and berry raw materials and secondary raw materials for the production of functional drinks. The optimal values for the cultivation of the studied cultures were established: pH – 6.5, temperature – for Zygosaccharomyces kombuchaensis sp. minimum growth temperature t = +15-20 °С, maximum – t = +45-50 °С, for Gluconoacetobacter xylinus (Brown 1886) Yamada et al 1998 minimum growth temperature t = +10- 25 °С, maximum – t = +45-50 °C; the content of reducing substances is 10-15 %. The data obtained will be used to develop biotechnological processes for the production of functional drinks.
In the present study the survival of the consortium of yeast and bacteria using protective media was investigated. The protective medium consisting of 1 % gelatin, 5 % monosodium glutamate and 5 % sucrose provides the best preservation of living cells of the consortium and allows stabilization of cells for up to 100 days at storage temperatures from -2 °C to -10 °C. The projective medium consisting of 1 % gelatin and 10 % sucrose provides effective storage of the cells at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 60 days. The projective medium of skimmed milk and 7.5 % glucose ensures effective storage of the cells of the consortium at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 80 days. The information obtained is interesting for following researches of the development of technologies for the bioconversion of plant materials.
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