Leclercia adecarboxylata (LAD) is a member of Enterobacteriaceae family that is usually reported as an opportunistic human pathogen. A few reports have described resistant strains in the literature. The aim of this paper was to describe the antimicrobial resistance pattern of a LAD strain isolated from a urinary tract infection in a 39-year-old immunocompetent man. The bacterial identification and antibiotic sensitivity tests were performed on Vitek 2 Compact 15. The results revealed the presence of LAD with a particular multidrug resistance pattern. It was sensitive only to imipenem (=1 µg/ml), and totally resistant to association of trimethoprim/sulfamethoxazole (≥320 µg/ml), ticarcillin (≥128 µg/ml), nitrofurantoin (=128 µg/ml), cefalothin (≥64 µg/ml), cefoxitin (≥64 µg/ml), cefotaxime (≥64 µg/ml), ceftazidime (≥64 µg/ml), amikacin (≥64 µg/ml), ampicillin (≥32 µg/ml), nalidxic acid (≥32 µg/ml), and a combination of amoxicillin/clavulanic acid (≥32 µg/ml), gentamicin (≥16 µg/ml), tobramycin (≥16 µg/ml), ofloxacin (≥8 µg/ml), and ciprofloxacin (≥4 µg/ml). It showed the intermediate sensitivity to the association of piperacillin/tazobactam (=64 µg/ml), and ertapenem (=4 µg/ml). The findings showed that this isolate of LAD had a multidrug resistance pattern to almost all the antibiotics tested (except imipenem). This suggests that LAD could be considered as an emergent bacterial pathogen capable of causing infections in human and carrying multidrug resistance pattern to numerous antibiotic families in Guinea.

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Introduction: Escherichia coli (E. coli) is one of the main bacterial species associated with urinary tract infections. Nowadays, this bacterium is becoming more and more resistant to antibiotics. Objective: The aim of this study was to determine the antibiotic sensitivity profiles of all strains of E. coli isolated from urine during the period from September 1st, 2018 to March 13th, 2019 at the Biomedical Laboratory of the China-Guinea Friendship Hospital of Kipé in Conakry. Materiel and Methods: Cultures were done on different agar media. Bacterial identification, antibiograms and determination of minimum inhibitory concentrations (MIC) were performed on the Vitek 2 Compact 15 automated system. Results: A total of 66 strains of E. coli have been isolated from patients of both sexes. The sex ratio (M/F) was 0.43. The mean age of the patients was 50.83 years. The majority of strains were sensitive to imipenem (96.96%), amikacin (96.96%), ertapenem (94.73%), gentamicin (69.23%), tobramycin (60, 60%), cefoxitin (64.28%), cefotaxime (62.50%), piperacillin/tazobactam (77.4%), amoxicillin/clavulanic acid (50.00%) and nitrofurantoin (87%). In contrast, the majority of strains were resistant to ampicillin (81.81%), cefalotin (62.02%), ticarcillin (88.00%), nalidixic acid (82.75%), ciprofloxacin (56.06%), ofloxacin (56.00%) and combination of trimethoprim/sulfamethoxazole (83.60%), sometimes with high MICs. Conclusion: Our results show that urinary tract infections due to E. coli are more frequently observed in females than in males. Some of these strains studied exhibited multidrug resistance profiles to antibiotics. Among the classes of antibiotics tested, carbapenemes, nitrofurans, aminoglycosides, appear to be more active on E. coli uropathogenes in Guinea.

Introduction: Diarrheal infections associated to multidrug resistant bacteria are a public health problem, particularly in the tropics. Objective: The aim of this study was to describe a Multidrug resistant strain of Escherichia coli (E. coli) isolated from diarrheal stools. Patients and methods: A sample of diarrheal stools from a 30 years old housekeeper patient was analysed at China-Guinea Friendship Hospital of Kipé/Conakry. Parasitological examination by optical microscopy, followed by bacteriological analysis were done. Cultures were carried out on different agar media. Bacterial identification, antibiograms and minimum inhibitory concentrations (MIC) were performed using the Vitek 2 System. Results: The isolated E. coli strain was sensitive only to 4 of 29 antibiotics tested including imipenem, ertapenem, amikacin and nitrofurantoin. Intermediate sensitivity was detected towards minocycline. In contrast, this strain was resistant to piperacillin, cefuroxime, cefuroxime axetil, cefixime, ceftriaxone, cefepime, aztreonam, meropenem, levofloxacin, ofloxacin, tetracycline, tigecycline, chloramphenicol, trimethoprim, ampicillin, amoxicillin/clavulanic acid, ticarcillin, piperacillin/tazobactam, cephalothin, cefotaxime, ceftazidime, gentamicin, tobramycin, nalidixic acid, ciprofloxacin, ofloxacin and trimethoprim/sulfamethoxazole with high MICs. Conclusion: The treatment of this multidrug-resistant Escherichia coli diarrheal infection requires appropriate antibiotic therapy, based on the results of an accurate antibiogram to be performed with rapid means for better patient care.

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