Volatile compounds from plants, callus tissue cultures, and cell suspensions of parsley (Petroselinum crispum) were captured during the growth cycle using a dynamic headspace extraction and were identified by gas chromatography-mass spectrometry. Parsley plants were found to produce mainly monoterpenes, and the compound of major abundance was p-1,3,8-menthatriene, followed by beta-phellandrene and apiole. Callus cultures and cell suspensions produced aldehydes (nonanal and decanal) that were also detected in parsley plant. The former also produced limonene, acetophenone, and benzotiazol; these were not observed in the plants. The production of volatiles in plants, callus tissue, and cell suspensions was found to be time-dependent. Free and bound fatty acids were also monitored by an in situ method. Palmitic (C16:0) and stearic (C18:0) acids were the most abundant fatty acids in all materials; however, higher levels were found in plants. On the other hand, the unsaturated C16:1 and C16:3 were not detected in the in vitro cultures.

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