Objectives A previous study reported that the hexane fraction (HF) obtained from Pereskia aculeata leaves showed promising topical anti-inflammatory activity. Intending its future use in clinical practice, a herbal medicine cream named INFLATIV was developed. Its anti-inflammatory and antipsoriatic potential were investigated. INFLATIV was subjected to preliminary accelerated stability tests and to a degradation profile assessment. Methods INFLATIV was prepared at 6% and 12%. The anti-inflammatory activity was assessed by croton oil single and multiple application challenge in mice. Mouse tail test was used for antipsoriatic potential investigation. Cutaneous atrophy test was performed. Preliminary accelerated stability tests were performed together with a degradation profile by GC-MS analysis. Key findings The anti-inflammatory activity shown by INFLATIV was comparable to dexamethasone. However, the skin atrophy caused by that drug was not observed. INFLATIV modified skin parakeratotic differentiation into orthokeratosis, which revealed its antipsoriatic potential. The ingredients used were suitable to carry the bioactives as they were well permeated by the skin. The preliminary accelerated stability tests indicated that INFLATIV 6% is more stable than 12%. Conclusions The results demonstrated the relevant therapeutic and marketing potentials of INFLATIV, which is likely to be further evaluated in clinical trials for drug registration process with regulatory agencies.
Pereskia aculeata Miller é uma trepadeira arbustiva popularmente conhecida como "orapro-nobis". Na medicina tradicional, as folhas são utilizadas como anti-inflamatórias, cicatrizantes e emolientes. O objetivo desse estudo foi investigar a constituição química e o potencial antioxidante de extratos glicólicos de P. aculeata. Durante dez dias, as folhas foram maceradas à temperatura ambiente com glicerina e álcool de cereais, sendo obtidos os extratos PA01 e PA02. Ao final do processo, o extrato PA01 foi aquecido a 50 °C por 30 minutos. Esta foi a primeira vez em que extratos de P. aculeata foram obtidos com os referidos solventes extratores. O teor de compostos fenólicos foi avaliado utilizando-se o reagente de Folin-Ciocalteau e o potencial antioxidante foi determinado pelo método de sequestro do radical DPPH. PA01 e PA02 apresentaram um teor de fenóis totais de 13,84 ± 4,29 e 18,35 ± 2,12 mg em equivalentes de ácido tânico/g de extrato, respectivamente. No método do DPPH, os valores de CI50 encontrados foram de 11,93 ± 1,84 e 9,91 ± 2,45 µg/mL, respectivamente, demonstrando uma boa atividade antioxidante. Além disso, o aumento da temperatura não foi um fator decisivo na extração, visto que o conteúdo fenólico e o potencial antioxidante foram estatisticamente semelhantes para ambos os extratos. O potencial antioxidante pode ser atribuído, pelo menos em parte, ao conteúdo fenólico, visto que PA01 e PA02 foram capazes de extrair esses compostos de forma semelhante. Portanto, os extratos glicólicos de P. aculeata apresentam potencial para contribuir com a prevenção de diversos distúrbios associados com a produção de radicais livres.
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