A laboratory study has successfully demonstrated that a nitrogen deficient thermomechanical pulping wastewater can be effectively treated in a sequencing batch reactor (SBR) operated under conditions of biological nitrogen fixation (the N-ViroTech process). In comparison to continuous stirred tank reactor activated sludge (CSTR-AS) configurations operated under either nitrogen fixing or nitrogen supplemented conditions, slightly lower removals of dissolved organic material were observed in the SBR. However, this was largely offset by significantly better suspended solids removal in the SBR, which contributes to the overall COD discharge. The settleability and dewaterability of sludge produced by the SBR was significantly better than that obtained from the nitrogen fixing CSTR-AS reactors, and comparable to that of a nitrogen supplemented system. Consistently low total and dissolved nitrogen discharges from the N-ViroTech systems demonstrated the advantage of this system over ones requiring nitrogen supplementation. The feast-famine regime of an SBR-type configuration has significant potential for the application of this technology in the treatment of nitrogen deficient waste streams, particularly those in which conventional single-stage systems may be susceptible to sludge bulking problems.

A BOD:N:P ratio of 100:5:1 is often used as a benchmark for nutrient addition in nutrient limited wastewaters. The impact of varying nitrogen levels, whilst maintaining phosphorus constant, was studied in a simulated aerated lagoon (BOD:N of 100:0; 100:1.3; 100:1.8; 100:2.7 and 100:4.9). A synthetic wastewater was prepared using methanol, glucose and acetate as the combined carbon source, ammonium chloride as the nitrogen source and dipotassium hydrogen phosphate as the phosphorus source. Nitrogen levels did not impact organic carbon removal, but did strongly influence floc structure. With no supplemental nitrogen, growth was dispersed. Increasing the nitrogen level increased filamentous growth, with a marked change in filamentous species occurring between a BOD:N ratio of 100:1.8 and 100:2.7. Nitrogen fixation occurred at a BOD:N ratio of 100:0; 100:1.3 and 100:1.8, with nitrogen loss at BOD:N ratios of 100:2.7 and 100:4.9. At a BOD:N ratio of 100:4.9, ammonium discharge was significantly greater (1.8 mg/L) than at the lower nitrogen levels (0.04 - 0.18 mg/L). Phosphorus behaviour was more variable, however significantly more phosphorus was discharged at the lowest nitrogen level than at the highest (p<0.05). Based on readily available nitrogen, the BOD:N ratio at which nitrogen fixation no longer occurred was around 100:1.9.

As pulp and paper wastewaters are mostly deficient in nitrogen and phosphorus, historical practice has dictated that they cannot be effectively treated using microbiological processes without the addition of supplementary nutrients, such as urea and phosphoric acid. Supplementation is a difficult step to manage efficiently, requiring extensive post-treatment monitoring and some degree of overdosing to ensure sufficient nutrient availability under all conditions. As a result, treated wastewaters usually contain excess amounts of both nutrients, leading to potential impacts on the receiving waters such as eutrophication. N-ViroTech is a highly effective alternative treatment technology which overcomes this nutrient deficiency/excess paradox. The process relies on communities of nitrogen-fixing bacteria, which are able to directly fix nitrogen from the atmosphere, thus satisfying their cellular nitrogen requirements. The process relies on manipulation of growth conditions within the biological system to maintain a nitrogen-fixing population whilst achieving target wastewater treatment performance. The technology has significant advantages over conventional activated sludge operation, including: Improved environmental performance. Nutrient loadings in the final treated effluent for selected nitrogen and phosphorus species (particularly ammonium and orthophosphate) may be reduced by over 90% compared to conventional systems; Elimination of nitrogen supplementation, and minimisation of phosphorus supplementation, thus achieving significant chemical savings and resulting in between 25% and 35% savings in operational costs for a typical system; Self-regulation of nutrient requirements, as the bacteria only use as much nitrogen as they require, allowing for substantially less operator intervention and monitoring. This paper will summarise critical performance outcomes of the N-ViroTech process utilising results from laboratory-, pilot-scale and recent alpha-adopter, full-scale trials.

Flow cytometry has potential as a rapid assessment technique to evaluate phytoplankton biomass and species composition. It facilitates for multi-parameter analysis of individual cells on the basis of light scattering effects induced from cellular constituents, as well as auto-fluorescence. Fluorescence emission characteristics may be especially useful in classifying cyanobacteria as they contain phycoerythrin which emits light predominantly in the 550-600 nm waveband, chlorophyll-a (650-700 nm emission) and allophycocyanin (660 nm emission). The objective of our study was to assess the utility of flow cytometry for the rapid identification and sorting of freshwater algae and cyanobacteria species. Using a selection of laboratory-cultured freshwater algae and cyanobacteria species, this study demonstrated unique light scatter and fluorescent characteristics for each species examined, allowing for rapid species identification and sorting of mixed populations of laboratory cultures and samples from two lakes in the Rotorua region (New Zealand). Analysis of lake water samples collected over seven months demonstrated changes in abundance and community composition of phytoplankton in the two lakes and demonstrates that flow cytometry may be a useful technique for examining seasonal changes in phytoplankton composition.

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