Trait abnormalities in bipolar disorder (BD) within ventral prefrontal cortex (vPFC) and amygdala suggest dysfunction in their connectivity. This study employed low frequency resting state functional magnetic resonance imaging (LFRS-fMRI) to analyze functional connectivity between ventral prefrontal cortex (vPFC) and amygdala in BD. LFRS-fMRI identified a negative correlation in vPFC-amygdala activity, and the magnitude of this correlation was greater in healthy participants than in subjects with BD. Additionally, whole brain analysis revealed higher correlations between left and right vPFC in BD, as well as with ventral striatum.