Introduction. So far, the most promising and most commonly used materials in endodontic treatment and retreatment were calcium silicate cements. However, due to the shortage of this material and treatment failures, a new bioactive material was introduced-Biodentine tm. It is a calcium silicate based technology, with excellent handling characteristics and biocompatibility. It can be used in various indications, including dentine substitution and endodontic therapy. Case reports. The clinical cases demonstrated excellent healing potential after the treatment with Biodentine tm. Conclusion. The bio-silicate technology is highly promising, mostly due to its chemical properties and easy clinical manipulation. The short setting time and high mechanical strength makes Biodentine tm a material easy to handle, highly biocompatible, with a wide range of indications.

Summary Esthetic and minimally invasive restorative techniques are current topics in dentistry. Introduction of modern composite materials and adhesive technology provided numerous options for design of esthetic restorations without invasive teeth preparation common for traditional prostodontic restorations. Modern esthetic dentistry can provide solutions for simple as well as complex cases by implementation of various alternatives.

BACKGROUND: Caries is a destructive process leading to progressive demineralization of the inorganic part of the tooth, accompanied by enzyme disintegration of the organic component of the tooth tissue. Considering the fact that caries activity can be stopped if the demineralization process is stopped, it is clear that the objectives of caries treatment are focused toward elimination of etiological factors and stimulation of regeneration of the dental tissues. That is why in the last years treatment of the caries disease is targeted to remineralization of the initial carious lesions, as well as on the biological behavior of the pulp-dentine complex after application of certain medications. AIM: The aim of this study is to evaluate impact of the two materials for direct capping, mineral trioxide aggregate (MTA) and Biodentine®, on the expression of glycoproteins fibronectin (FN), and tenascin (TN), responsible for dentinogenesis. METHODS: In the tests were used materials MTA and Biodentine, as agents for direct capping of pulp exposure. From 60 extracted teeth included in this in vitro study, tissue cuts were made. Each of them was then analyzed on a light microscope to determine the amount of two extracellular matrix glycoproteins, FN-C, and TN-C. RESULTS: Our study show that there is an expressed immunoreactivity for FN and for TN in the fibronectin bridge under MTA and Biodentine® after 8, and even after 30 days of their application. CONCLUSION: Based on this study, we can conclude that both materials – MTA and Biodentine – may induce reparative dentinogenesis, in which FN and TN have certainly a major role in the formation of the fibronectin matrix.

Primary purpose of restorative dentistry is to preserve pulp vitality. Besides calcium hydroxide, the application of calcium silicate cements as a material for direct pulp capping has become used recently. The aim of our study was to investigate the influence of materials for direct and indirect pulp capping on the bond strength of composite restorations using two different self etch dentin adhesives. The test was performed on 60 intact molars extracted for periodontal or orthodontic reasons. The prepared samples were divided into the two groups and three subgroups depending on the adhesive and pulp cupping material. Two different calcium silicate based materials were used for pulp cupping. One step and two steps self etch dentin adhesive was applied to prepared specimens depending on the group and with the help of a specially made metal mold set up a composite post. The share bond strength was assessed using a universal testing machine. The results showed significant difference in the share bond strength between the samples treated with TheraCal LC and Biodentine with TheraCal LC being superior to Biodentine.

Summary Intoduction Pain and discomfort during dental interventions caused by high-speed dental burs are the most frequent reasons for avoiding dental visits. Numerous possibilities have been offered in the last decade aiming to replace burs for caries removal, one of them is chemo-mechanical method of caries elimination. The aim of this study was to analyze micromorphological changes caused by Carisolv on sound, demineralized and carious (softened) dentin using SEM. Material and Methods Study included 30 teeth (20 intact and 10 with carious lesions) extracted for orthodontic reasons. Carisolv™ system (MediTeam, Sweden) that contains Carisolv gel and specific nickel-titanium hand instruments was used for chemo-mechanical caries elimination. In our study only Carisolv gel was applied on sound, demineralized and carious dentin for 20 min. Dentin surface was analyzed using scanning electronic microscope (SEM). Results SEM showed that Carisolv gel affected soft carious dentin only with no changes on sound dentin regardless of demineralization status. Conclusion Carisolv did not affect sound and demineralized dentin while it selectively dissolved carious dentin.