The final consistence obtained in MTA Flow may facilitate several procedures, indicating that the MTA Flow has a promising application in endodontics.

Marina Tolomei Sandoval CURY (a) Ana Maria Veiga VASQUES (a) Jimena Lama SARMIENTO (a) Juliana Quintino TRIZZI (a) Rogério Castilho JACINTO (a) Gustavo SIVIERI-ARAUJO (a) Eloi DEZAN JÚNIOR (a)

The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction induced by Psidium cattleianum leaf extracts associated with Ca(OH) 2 . Thirty male Wistar rats, split equally into three groups [aqueous extract + Ca(OH) 2 ; ethanolic extract + Ca(OH) 2 ; and propylene glycol + Ca(OH) 2 ], were assessed every 3 h or 6 h (five animals in each period). Under general anesthesia, 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein and each combination to be evaluated was subcutaneously injected into the dorsal region 30 min thereafter. Edemogenic activity was analyzed by spectrophotometry (λ=630 nm). For inflammatory reaction analysis, 50 rats received four polyethylene tubes (three experimental groups) and an empty tube (control group). The assessments were made at 7, 15, 30, 60, and 90 days, followed by hematoxylin-eosin staining and by the assignment of scores for evaluation of tissue response intensity. Ethanolic extract + Ca(OH) 2 yielded the largest edemogenic activity at 3 h. Intergroup differences at 6 h were not significant. The histological analysis showed progressive repair over time (p<0.05) and aqueous and ethanolic extracts produced similar responses to those of the control and Ca(OH) 2 + propylene glycol groups. Psidium cattleianum leaf extracts used as Ca(OH) 2 vehicles evoked similar tissue response when compared to Ca(OH) 2 associated with propylene glycol.

Marina Tolomei Sandoval CURY (a) Ana Maria Veiga VASQUES (a) Gustavo SIVIERI-ARAÚJO (a) Rogério Castilho JACINTO (a) João Eduardo GOMES-FILHO (a) Luciano Tavares Angelo CINTRA (a) Eloi DEZAN-JÚNIOR (a)

This research evaluated, in vivo, the accuracy of three electronic apex locators -EALs (Root ZXII, E-PEX and FIND) in teeth with vital pulp submitted to biopulpectomy, preserving the periodontal stump. For this study, 90 single-rooted teeth with extraction indication were selected. After positive pulpal cold sensitivity test, pulp chamber access was performed. The cervical and middle thirds of root canals were instrumented with Reciproc R25, and the K#15 file was used as a standard instrument to determine working length, forming 2 groups: Constriction (insertion of the instrument until the apical constriction limit) and Foramen (insertion of the instrument until the foramen and then repositioning at constriction, without removing the file from the canal). The hand file was stabilized with a light-cured flow resin. After extraction, the samples were analyzed through microCT SkyScan 1272, with CTAN software, which evaluated the proximity between the tip of the file to the apical constriction, providing data for comparative analysis using Kruskal-Wallis and Dunn tests (p<0.05). There was a statistically significant difference in the abilities of the EALs to detect the apical constriction after reaching the foramen with Root ZX II showing higher accuracy (89%). However, there was no difference in the accuracy of the three EALs in detecting the apical constriction without reaching the foramen. Based on the present results, we conclude that EALs may show accurate measures in detecting apical constriction and foramen, even without damaging the periodontal stump in biopulpectomy.