Enforcement mechanisms are thought to be important in maintaining mutualistic cooperation between species. A clear example of an enforcement mechanism is how legumes impose sanctions on rhizobial symbionts that fail to provide sufficient fixed N 2 . However, with domestication and breeding in high-soil-N environments, humans may have altered these natural legume defences and reduced the agricultural benefits of the symbiosis. Using six genotypes of soya beans, representing 60 years of breeding, we show that, as a group, older cultivars were better able to maintain fitness than newer cultivars (seed production) when infected with a mixture of effective and ineffective rhizobial strains. Additionally, we found small differences among cultivars in the ratio of effective : ineffective rhizobia released from their nodules, an indicator of future rhizobial strain fitness. When infected by symbionts varying in quality, legume defences against poor-quality partners have apparently worsened under decades of artificial selection.

Bell peppers (Capsicum annuum) are an economically important yet difficult to grow crop in northern New England. Yields of bell peppers can be increased through the use of plastic mulches; however, refinements are needed to make bell peppers a more viable crop in regions with short, variable growing seasons. The objectives of this study were to (1) compare the effects of black mulch with white inter-row much, reflective silver mulch, and standard black plastic mulched beds on bell pepper yield and quality and (2) compare the effects of two in-row plant arrangements [single rows at 12-inch within-row spacing (7260 plants/acre) and double rows spaced 18 inches apart with 18-inch in-row spacing (9680 plants/acre)] on pepper yield and quality. Treatments were factorial combinations of three mulch treatments and two within-row planting arrangements. Double rows produced more fruit by number and weight than single rows; however, fruit harvested from the double-row plots tended to be smaller than fruit harvested from the single-row plots. Mulch treatments significantly influenced total marketable yield and yield of cull bell peppers grown in Maine. The plots receiving the inter-row white mulch or reflective silver mulch treatment produced significantly greater yield than standard black plastic mulch treatment. The reflective mulch treatment produced significantly more cull fruit per acre compared with the white inter-row mulch and black plastic.

Intensive tillage degrades soil structure, decreases soil organic matter, and can cause soil compaction and erosion over time. Organic vegetable farmers are often dependent on tillage to incorporate crop residue, control weeds, and prepare seedbeds. Black, impermeable, polyethylene tarps applied on the soil surface and removed at planting can help suppress weeds before planting and reduce farmers’ reliance on tillage. However, little is known about how black tarps affect planting conditions and how they can be used to advance reduced tillage production systems. This study investigated the effects of tarp use and tarp duration on the soil environment, surface cover crop residue, and weed suppression to assess the efficacy of using tarps to improve reduced- and no-till practices for organic vegetable production. Experiments were conducted at three sites in the northeastern United States (Freeville, NY; Riverhead, NY; and Monmouth, ME) for 2 years. Following the termination of an oat cover crop, tarps were applied over untilled soils and left in place for four time periods: untarped (control), 3 to 5 weeks (short), 6 to 8 weeks (mid), and 10 or more weeks (long) before two removal dates. Soil moisture and temperature, cover crop residue, soil inorganic nitrogen, weed seed survival, and weed percent cover were measured after tarp removal. Soil moisture and temperature were generally higher under tarps at the time of removal compared with untarped areas at 10% to 55% and 1 to 3 °C, respectively, but the effects were inconsistent. Tarps significantly increased soil nitrate concentrations by 2-times to 21-times with longer tarp durations, resulting in higher concentrations compared with untarped controls. Tarps did not affect the amount of soil covered by cover crop residue and had no consistent effects on weed seed survival of Amaranthus powellii S. Wats. or Chenopodium album L., two common annual weed species in the Northeast. Tarping for at least 3 weeks reduced the weed percent cover by 95% to 100% at the time of removal. Increasing tarp duration beyond 3 weeks did not affect any measures except soil nitrate concentrations. These results indicate that tarps can facilitate the use of reduced-till and no-till practices for organic vegetables by creating a nutrient-rich and moist soil environment free of emerged weeds before planting without soil disturbance.

To determine if present commercial broccoli cultivars meet the diverse needs of organic management systems, such as adaptation to low N input, mechanical weed management, and no chemical pesticide use, and to propose the selection environments for crop improvement for organic production, we compared horticultural trait performance of 23 broccoli cultivars (G) under two management (M) systems (organic and conventional) in two regions of the United States (Oregon and Maine), including spring and fall trials. In our trials, location and season had the largest effect on broccoli head weight, with Oregon outperforming Maine, and fall trials outperforming spring plantings. M main effects and G × M interactions were often small, but G × M × E (location and season) were large. Cultivars with both greater head weight and stability under conventional conditions generally had high head weight and stability under organic growing conditions, although there were exceptions in cultivar rank between management systems. Larger genotypic variances and somewhat increased error variances observed in organic compared with conventional management systems led to repeatability for head weight and other horticultural traits that were similar or even higher in organic compared with conventional conditions. The ratio of correlated response (predicting performance under organic conditions when evaluated in conventional conditions) to direct response (predicted performance in organic when evaluated under organic conditions) for all traits was close to but less than 1.0 with the exception of bead uniformity. This would imply that in most cases, direct selection in an organic environment could result in a more rapid genetic gain than indirect selection in a conventional environment.