The overall goal of this project was to quantify the long-term water quality impacts of land management changes associated with increased demands for corn as a transportation biofuel feedstock in the United States. A modeling approach that considers a nonpoint source model, Groundwater Loading Effects of Agricultural Management Systems and National Agricultural Pesticide Risk Analysis, was used to simulate annual losses in runoff, percolation, erosion, nitrate-nitrogen, total phosphorus, atrazine ͑1-chloro-3-ethylamino-5isopropylamino-2,4,6-triazine͒, and pyraclostrobin ͑Methyl ͕2-͓1-͑4-chlorophenyl͒-1H-pyrazol-3-yloxymethyl͔ phenyl͖ methoxycarbam-ate͒ to the edge-of-field and bottom-of-root zones associated with multiple cropping scenarios. Model results for representative soils, throughout Indiana, were analyzed to determine 10% ͑worst case͒ and 50% ͑average case͒ probability of exceedence in the aforementioned water quality indicators. Modeling results indicated significant differences ͑p Ͻ 0.05͒ in water quality indicators between continuous corn and corn-soybean rotations. The results showed that agricultural management decisions would have greater impacts on nutrient, runoff, erosion, and pesticides losses from agricultural fields compared to water quality indicators associated with the projected changes in crop rotation systems. The model results point to the need for additional research to fully understand the water impacts of land management decisions associated with corn grain as a feedstock for biofuel production.

Thiamin and riboflavin concentrations in urine and breast milk were measured to see if vitamin supplementation during lactation is beneficial to healthy well-nourished women. seven supplemented subjects and five nonsupplemented subjects expressed milk four times per day for 3 days at 1 and 6 weeks postpartum. Dietary intakes were recorded for 1 day before milk collection and the 3 days of milk collection. A 24-hr urine sample was collected at 1 and 6 weeks postpartum. Mean thiamin concentration in the milk increased significantly in both groups between 1 and 6 weeks postpartum, although there were no significant differences between groups. Riboflavin values reflected in the milk were significantly lower in the nonsupplemented group of women both at 1 and 6 weeks postpartum, but values did not change significantly in either groups as the milk matured from 1 to 6 weeks postpartum. Urinary excretion of thiamin and riboflavin was proportionally higher in the supplemented group of women. Riboflavin and thiamin status of both groups of women depicted normal intakes and the milk concentration of thiamin and riboflavin either met or exceeded established norms in both groups of women.

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In this double-blind study, the effects of ascorbic acid, vitamin B6, vitamin B12, and folate supplementation on the maternal nutritional status and vitamin content of breast milk in 16 low socioeconomic lactating women were studied. Nine subjects were administered a commercial multivitamin-multimineral supplement and seven were given placebos. Milk samples were collected four times per day at 4-h intervals from 5 to 7 and 43 to 45 days postpartum. Fasting blood samples were taken from all mothers at the end of each milk collection period. Dietary records of all foods consumed were kept from 4 to 7 and 42 to 45 days postpartum. There were no significant differences in milk or plasma levels of ascorbic acid between the unsupplemented and supplemented groups. Both the EGPT index and milk concentration of vitamin B6 were significantly different (p less than 0.01) between the unsupplemented and supplemented groups. The milk concentrations of vitamin B12 increased significantly (p less than 0.05) in the supplemented group, as did the milk concentrations of folate (p less than 0.01). Because of consistently low levels of dietary vitamin B6 and folic acid in this group of low socioeconomic lactating women, either dietary changes or supplements could be necessary to maintain recommended levels of these vitamins in the womens' breast milk.