Purpose: This study investigates the antiangiogenesis and antitumor efficacy of a recombinant protein composed of the three type 1 repeats (3TSR) of thrombospondin-1 in an orthotopic human pancreatic cancer model and provides useful preclinical data for pancreatic cancer treatment.Experimental Design: Human pancreatic cancer cells (AsPC-1) were injected into the pancreas of severe combined immunodeficient mice. The animals were treated with 3TSR (3 mg per kg per day) or PBS for 3 weeks. Subsequently, the effects of 3TSR on tumor growth, microvessel density, cancer cell proliferation, apoptosis, and endothelial cell apoptosis were analyzed. The in vitro effects of 3TSR on human pancreatic cancer cells were also studied.

B lymphocyte stimulator (BLyS) is a tumor necrosis factor (TNF) family member and a key regulator of B cell responses. We employed a phage display-based approach to identify peptides that bind BLyS with high selectivity and affinity. Sequence analysis of first-generation BLyS-binding peptides revealed two dominant peptide motifs, including one containing a conserved DxLT sequence. Selected linear peptides with this motif were found to bind BLyS with K(D) values of 1-3 microM. In order to improve the binding affinity for BLyS, consensus residues flanking the DxLT sequence were seeded into a second-generation, BLyS affinity maturation library (BAML). BAML phage were subjected to stringent binding competition conditions to select for isolates expressing high-affinity peptide ligands for BLyS. Post-selection analysis of BAML peptide sequences resulted in the identification of a core decapeptide motif (WYDPLTKLWL). Peptides containing this core motif exhibited K(D) values as low as 26 nM, approximately 100-fold lower than that of first-generation peptides. A fluorescence anisotropy assay was developed to monitor the protein-protein interaction between BLyS labeled with a ruthenium chelate, and TACI-Fc, a soluble form of a BLyS receptor. Using this assay it was found that a BAML peptide disrupts this high-affinity protein-protein interaction. This demonstrates the potential of short peptides for disruption of high affinity cytokine-receptor interactions.

In this paper, the research results of the stability of steel welded tubes forming process are presented. The aim of this research is to determine influence of geometrical and tribological parameters on stability of the process and to determine optimal values of influential process parameters. A research plan with variation of influential parameters was made, on the basis of which experimental and numerical experiments were performed. Tube forming was performed in one operation in a two-part tool made of hard metal by a combination of widening and narrowing. The geometrical factors observed during experiments are length, outer diameter and thicknesses of steel welded tube as a billet. Friction conditions in contact between tool and tubular workpiece are considered in two cases, the first one when standard machine oil is used as a lubricant and the second one when tube billet surface is phosphated. Based on results of experiments, influencing factors on stability of tube forming process were analysed and optimal production technology was recommended, including optimal values of influencing factors. Results obtained by experimental research were confirmed through numerical experiments based on finite element method.

U ovom radu je prikazana ušteda u energetskom inputu, proizvodnje i raspodele mineralnih hraniva, korišćenjem ostatka fermentacije - digestata.

The application of numerical simulations of production technologies, using the finite element method or the finite volume method, is a widely used approach in advanced engineering design and research. Unlike industrial experiments, this approach allows the analysis of the influence of different process parameters on the output performance of a workpiece. The paper analyzes the influence of the choice of the machine on the forging process of the connecting rod, which is made, among other things, for the needs of the auto industry. For this purpose, numerical experiments were carried out, using the finite volume method and the Simufact.forming software package, with variations of three types of forging machines: drop forging hammer, crank press and screw press. Finally, the results of the numerical experiments and conducted analyses are presented, with concluding considerations and recommendations.