Traditional aerobic exercise decreases the risk of developing inflammatory diseases in both obese and normal weight individuals. The magnitude of the anti‐inflammatory immune response following traditional exercise is reduced and the time‐course is shorter in obese compared to normal weight individuals. Although obesity may affect the ability to engage in traditional exercise, whole body vibration (WBV) has emerged as a more tolerated alternative. The impact of WBV on immune‐mediated inflammation in obesity, however, is unknown. PURPOSE To determine if WBV elicits a differential magnitude and time‐course of immune‐mediated inflammation between obese and normal weight individuals. METHODS 11 obese (OB) (BMI: 35.9 ± 8.8 kg/m2, Age: 33 ± 4 y, percent body fat (%BF): 39.1 ± 2.4%) and 12 normal weight (NW) (BMI: 21.3 ± 2.2 kg/m2, Age: 29 ± 7 y, %BF: 17.4 ± 2.1%) men and women participated in this study. Following IV insertion and a resting (PRE) blood draw, each participant performed the WBV protocol, which consisted of 10 cycles of 1 minute of WBV in a static squat position followed by 30 seconds of rest. Following completion of the WBV protocol, blood samples were obtained immediately (POST), 1 hour (1h), 3 hours (3h), and 24 hours (24h) post‐WBV. Blood was analyzed for leukocyte composition (immune‐mediated inflammation) and IL‐6 (muscle activation). RESULTS PRE leukocyte composition was not different between groups. In NW, the percentage of neutrophils decreased (1h: 62 ± 3% vs. 24h: 54 ± 3%, p=0.009) and the percentage of lymphocytes increased (1h: 28 ± 3% vs. 24h: 35 ± 2%, p=0.011). In contrast, there were no changes in leukocyte composition in OB. An association was observed between %BF and the percentage of monocytes at POST (r= −0.67, p=0.023) and 1h (r= −0.65, p=0.03) in OB, whereas in NW %BF was only correlated with percentage of monocytes at PRE (r= −0.61, p=0.045). Although IL‐6 was higher in OB compared with NW at each time point (all p<0.05), a similar percent change from baseline was observed between groups for all post‐WBV time points. IL‐6 at PRE was correlated with percent change at 3h (r= −0.61, p=0.048) only in OB. CONCLUSION WBV elicited favorable alterations in leukocyte composition over time in NW, while no change in leukocytes were detected in OB. In addition, adiposity appears to impact the timing of the leukocyte response to WBV. Although WBV provoked similar IL‐6 changes in both groups, higher concentrations of IL‐6 at PRE appeared to be related to smaller changes at 3h in OB. Taken together, these data suggest a differential inflammatory immune response to WBV exercise between obese and normal weight individuals. Support or Funding Information Supported by Medical College of Georgia TUPP award This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Adverse childhood experiences (ACEs) are traumatic events during the first years of life that are associated with a higher risk of developing cardiovascular disease (CVD) during adulthood. The medial prefrontal cortex (mPFC) is a core region in the brain that modulates emotions and is directly involved in the cardiovascular response to stress by increasing vascular resistance. In the present study we examined the relationship between ACEs, mPFC and peripheral vascular function. Forty-five, adults (33±5 yrs.) participated in the present study to evaluate cerebral hemodynamics and peripheral vascular function. The impact of adverse experiences was evaluated through the ACE questionnaire. Among those that experienced ACEs (ACE group, n = 22), there was a significantly (P&lt;0.001) reduced activation of the mPFC as well as greater peripheral vascular resistance observed in the small (P ≤ 0.035), conduit (P ≤ 0.042) and large (P ≤ 0.001) blood vessels, when compared to those that did not report ACEs (Control group, n = 23). In addition, relationships between the number of ACEs and mPFC activation (rs = -0.428; P = 0.003) and peripheral vascular function (rs≤-0.373; P ≤ 0.009) were observed. Findings from the present study support that adults who experienced ACEs exhibit a reduced activation of the mPFC along with systemic vascular dysfunction. In addition, individuals exposed to more childhood traumatic events exhibited a progressively greater inactivation of the mPFC and an increased peripheral vasoconstriction in a dose-dependent manner. These findings provide novel insights into the potential role that the brain and the peripheral vasculature may have in connecting adverse childhood events to the increased risk of CVD.

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