The aim of this study was to evaluate the kinetic parameters and the production of metabolites of 13 novel yeasts isolated from a distillery and fruits, and Saccharomyces cerevisiae CAT-1, cultivated in fructose-based medium. The yeasts with the highest µ max were obtained from must, Pichia kudriavzevii BB2, P. kudriavzevii BB1, and S. cerevisiae BB9 (0.47-0.49 h −1). S. cerevisiae CAT-1 (3.02 g gDCM −1 h −1), S. cerevisiae BB9 (3.01 g gDCM −1 h −1), and Candida glabrata Recol 41 (2.52 g gDCM −1 h −1) stood out in terms of µ S. C. parapsilosis Recol 29, and Rhodotorula mucilaginosa Recol 03 strains showed the highest Y X/S (0.30 and 0.28 gDCM g −1 , respectively). C. glabrata Recol 10 and S. cerevisiae BB9 strains stood out for their higher substrate conversion rates into ethanol (0.44 and 0.41 gEth gS −1 , respectively). R. mucilaginosa Recol 03 presented the poorest performance in substrate consumption (0.87 g gDCM −1 h −1), while the strains isolated from must and C. glabrata Recol 10 showed the highest ethanol production and the C. parapsilosis Recol 29 showed the highest biomass conversion.

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