Physiology of yeast strains isolated from Brazilian biomes in a minimal medium using fructose as the sole carbon source reveals potential biotechnological applications

Abstract

The aim of this study was to evaluate the kinetic parameters and the production of metabolites of 13 novel yeasts isolated from a distillery and fruits, and Saccharomyces cerevisiae CAT-1, cultivated in fructose-based medium. The yeasts with the highest µ max were obtained from must, Pichia kudriavzevii BB2, P. kudriavzevii BB1, and S. cerevisiae BB9 (0.47-0.49 h −1). S. cerevisiae CAT-1 (3.02 g gDCM −1 h −1), S. cerevisiae BB9 (3.01 g gDCM −1 h −1), and Candida glabrata Recol 41 (2.52 g gDCM −1 h −1) stood out in terms of µ S. C. parapsilosis Recol 29, and Rhodotorula mucilaginosa Recol 03 strains showed the highest Y X/S (0.30 and 0.28 gDCM g −1 , respectively). C. glabrata Recol 10 and S. cerevisiae BB9 strains stood out for their higher substrate conversion rates into ethanol (0.44 and 0.41 gEth gS −1 , respectively). R. mucilaginosa Recol 03 presented the poorest performance in substrate consumption (0.87 g gDCM −1 h −1), while the strains isolated from must and C. glabrata Recol 10 showed the highest ethanol production and the C. parapsilosis Recol 29 showed the highest biomass conversion.