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The triple-to-double coincidence ratio (TDCR) method is a liquid scintillation primary method for the absolute activity measurement of pure β− and pure electron capture emitters. This method requires specific three-photomultiplier liquid scintillation counters. The aim of the present work is to assess the TDCR method performance for routine tritium analysis in urine using an HIDEX 300 SL, the only three-photomultiplier liquid scintillation counter designed for routine laboratories. The physical parameters and the semi-empirical Birks parameter (kB) of the prepared liquid scintillation source were firstly determined. TDCR model equations solving and detection efficiencies calculations for measured samples were performed by TDCR07c computing program. Accuracy, uncertainties and detection limit of TDCR method were assessed through the tritium analysis of six intercomparison urine samples. The results demonstrate that the analytical performance of the TDCR method implemented on the HIDEX 300 SL is conform to the recommendations for the monitoring of workers exposed to tritium.