Two trisomic-21 (T-21), one monosomic-21 (M-21), and three diploid (D-21) human fibroblast cell strains were compared for their sensitivity to the antiviral and anticellular activities of human type I (HuIFN-P), human type I1 (HuIFN-y), murine type I (MuIFN-P), and murine type I1 (MuIFN-y) interferons. Antiviral activity was monitored by the reduction of vesicular stomatitis virus yield and anticellular activity was assessed by the inhibition of [methyl-3H]thymidine uptake in proliferating cells. T-21 cells were more sensitive to the antiviral activities of HuIFN-P and MuIFN-P than either D-21 or M-21 cells. However, T-21 cells were not more sensitive to the antiviral activities of HuIFN-y and MuIFN-y. Neither did T-21 cells exhibit an increased sensitivity to the anticellular activity of either HuIFN-/3 or -7, or MuIFN-/3 or -y. A chromosome 21 dosage effect could not be observed under any of our test conditions. 103 0037-9727/82/050103-09$01 .OO/O

2‐5A[ppp(A2'p) n 5'A] has been implicated as a mediator in the antiviral action of interferon. Its direct evaluation as an indicator of virus replication is hampered by two limitations: its inability to penetrate intact cells, and its rapid intracellular degradation by (2'–5')phosphodiesterase. These problems could be overcome by using a microinjection technique whereby a phosphodiesterase‐resistant analog of 2‐A, in which the 2'‐terminals adenosine residue is replaced by 2‐(9‐adenyl)‐6‐hydroxy‐methyl‐4‐hexylmorpholine, was injected into individual HeLa cells before infection with mengovirus or vesicular stomatitis virus (VSV). This comparative assay with two representatives of different virus classes in a single experimental system pointed to the high sensitivity of VSV to inhibition by 2‐5A oligonucleotides, in contrast with the low sensitivity of mengovirus. Microinjection of the hexylmorpholine 2‐5A analog led to a much greater reduction in mengovirus yield than did microinjection of 2‐5A itself.

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