JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org. Abstract. A survey was made in the SonoranDesert of yeasts living in the decaying arms of five species of cereus cacti and the four species of Drosoplhila that utilize them as host plants. The most common yeasts among 132 isolates from the cacti and 187 isolates from the flies, respectively, were: Pichia membranaefaciens (45% and 67%), Candida ingens (17% and 4%), Torulopsis sonorensis (12% and 11%), and Cryptococcus cereanus (8% and 7%). Eighty-eight percent of the 66 initial isolates of P. membranaefaciens from Drosophila pachea and its host, senita cactus, assimilated D-xylose while only 12% of the remaining 257 initial isolates did so. Nineteen of the 20 isolates of T. sonorensis from flies were found in Drosophila mojavensis and 12 of the 14 isolates of C-yptococcus cereanus came from D. pachea. The highest mean number of yeast species per cactus was 2.77 ? 0.68 in organpipe cactus and per fly was 1.63 + 0.53 in D. pachea. The flies usually carried fewer yeast species than were found in the host plant but D. pachea had almost the same mean and variance as its host, senita cactus, which had 1.64 ? 0.40. Yeast species diversity and average niche overlap have the following rank order among habitats and localities: temperate trees > temperate flies > tropical flies > desert cacti > desert flies. Habitat diversity and average niche width show: tropical flies > temperate flies and desert cacti > desert flies and temperate trees. The physiological properties of the desert yeasts are most similar to those of the tropical yeast. However, desert yeasts have similarities with yeasts of temperate trees. Both have low fermentative ability and high assimilation ability of several alcohols and acids. The genus Pichia is by far the most common yeast genus associated with Drosophila in all habitats analyzed (36% of 1,426 isolates).

A survey was made of the yeast communities isolated from necrotic tissue of 4 species of prickly-pear cacti (Opuntia stricta, O. tomentosa, O. monacantha, andO. streptacantha) which have colonized in Australia. Yeast communities were sampled from a number of localities and at different times. Cactus specific yeasts accounted for 80% of the total isolates, and the 3 most common species contributed 63% of the total. Comparisons of the species compositions of the yeast communities indicated that the differences among communities were greater betweenOpuntia species than between different localities within a single cactus species, and also that differences between years were greater than average differences between localities within years. Multivariate statistical tests of association between yeast community and physical features of rots indicated that temperature, pH, and age of rot all exerted some influence on the structure of the yeast community. Similar analyses involvingDrosophila species inhabiting these cactus rots suggested the existence of complex associations betweenDrosophila community, yeast community, and physical and chemical attributes of the cactus necroses.

of the yeast flora inthe breeding sites of cactophilic Drosophila. Can. J. Microbiol. 29: 6-14. Yeasts were isolated from 278 rots in cladodes and basal stems of Opuntia stricta sampled over 15 months at one locality. The most common yeast species among 370 nonidentical isolates representing 20 species were Candida sonorensis (27% of isolates) and Pichia cactophila (24%). These species generally were present in all months and throughout the area sampled. Other relatively common species (3-9% of all isolates) had much more restricted distributions in both time and space. The mean number of yeast species per rot was 1.27 k 0.082. and no yeasts were isolated from 20% of the rots sampled. Significant differences among months or seasons in average number of yeast species per rot were due primarily to lower numbers in the summer and winter months, while differences among rot types resulted mainly from a higher number in the longer persisting basal stem rots. Both hierarchical diversity analysis and analysis of the distributions of the 10 most common yeast species showed temporal effects (month or season) to be the major component contributing to heterogeneity, while microgeographic effects and rot type also were significant. The seasonal and microgeographic heterogeneity are interpreted in terms of longer persisting rots in part of the locality providing a reservoir for yeast species through the winter and are discussed in relation to the maintenance of genetic variation in cactophilic Drosophila that utilize the rots as feeding and breeding sites.

Four yeast strains, causally associated with infection in a patient with acute myelogenous leukemia, were identified by standard methods currently used in yeast taxonomy as representatives of Candida lusitaniae van Uden et do Carmo-Sousa. Because this species has not been recognized previously as an opportunistic yeast in humans, molecular taxonomic methods were applied to confirm its identity. The nuclear deoxyribonucleic acid (DNA) base composition of two clinical isolates was shown to be 45.1 mol% guanine plus cytosine as compared to 44.7 mol% guanine plus cytosine for the type strain of this species. DNA/DNA reassociation experiments revealed more than 95% complementarity between the DNAs from the clinical isolates and that of the type strain of C. lusitaniae, thus confirming their classification by conventional taxonomy. A key is provided to differentiate C. lusitaniae from two phenotypically similar Candida species.

Yeasts were isolated from the rotting stems of 7 species of cereoid cacti and 4 species ofDrosophila which utilize them as host plants. The yeast most common among 132 nonidentical isolates from the cacti and 187 nonidentical isolates from the flies, respectively, were:Pichia membranaefaciens (59 and 126),Candida ingens (22 and 8),Torulopsis sonorensis (16 and 20), andCryptococcus cereanus (11 and 14). Isolates capable of utilizingD-xylose were recovered primarily fromD. pachea andL. schotti. Adult flies were present on the substrates whenP. membranaefaciens was at high concentrations. As the pH of the substrates increased, the percent ofC. ingens cells increased relative to other yeast species. Larvae were detected mainly in alkaline substrates, and since adults did not yieldC. ingens to the extent the substrates did,C. ingens may be important in larval nutrition.Torulopsis sonorensis was recovered mainly fromD. mojavensis and its host plants,M. gummosus andL. thurberi. The concentration ofT. sonorensis in the substrates was negatively correlated with the temperature of the substrate.Cryptococcus cereanus was found in high concentrations in suitable tissues for adult flies but most adults did not yield this species to any extent. The yeast habitat diversities from the substrates had the following order:L. thurberi > C. gigantea > C. gigantea soils ≫ M. gummosus > L. schotti > others. Habitat diversity is discussed in relation to the variation of the physical conditions and chemical composition of the substrates. The yeast habitat diversities from the flies had the orderD. pachea > D. mojavensis ≫ D. nigrospiracula > undescribed Species M. The degree of habitat diversity is possibly a function of the surface feeding behavior of the flies.