Abstract. The present study evaluated the proteomic profile of saliva and plasma from women with impalpable breast lesions using nano-liquid chromatography-quadrupole-time-of-flight (nLC-Q-TOF) technology. Plasma and saliva from patients with fibroadenoma (n=10), infiltrating ductal carcinoma (n=10) and healthy control groups (n=8) were assessed by combinations of inter/intra-group analyses, revealing significant quantitative and qualitative differences. The major differentially-expressed proteins in the saliva of patients compared with the controls were α2-macroglobulin and ceruloplasmin, but the proteins that met the minimum fold-change and P-value cut-offs were leukocyte elastase inhibitor and α-enolase, and deleted in malignant brain tumors 1. Concerning plasma, α-2-macroglobulin and ceruplasmin were upregulated, while other proteins such as haptoglobin, hemopexin and vitamin D-binding protein were downregulated compared with the control. The changes in immune, molecular transport and signaling pathways were the most representative in the proteomic profile of the saliva and plasma. This is the first study to describe the proteome of saliva and plasma from the same women with impalpable breast lesions.

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Gas station workers are exposed to chemicals known to be carcinogenic, especially benzene. The objective was to analyze the health problems of female gas station workers by means of sociodemographic and clinical questionnaires, and laboratorial exams. We performed the genotyping of the polymorphisms BRCA1/P871L and BRCA1/Q356R by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, and of variant allele BRCA2/N372H through direct sequencing. The female workers showed a higher concentration of monocytes (P = 0.039); a greater number of spontaneous abortions (P = 0.025, OR = 4.977, 95% CI = 1.135-30.669); higher tobacco consumption (P = 0.013); and higher alcohol consumption (P = 0.05). The statistical analysis of the polymorphisms associated with the variables monocyte concentration and miscarriage number did not reveal a significant relationship, and smoking and spontaneous abortion were not statistically associated either. Environ. Mol. Mutagen. 58:730-734, 2017. © 2017 Wiley Periodicals, Inc.

Background: Brazilian gas station workers (GWS) are daily exposed to petroleumderived hydrocarbons which are harmful. The aim of the study: The aim of this study was to evaluate the clinical complaints and the genotoxic effects in GWS. Materials and methods: Overall, 88 workers were recruited versus 127 controls. To test the influence of the time of exposure, we have divided the workers into two groups arbitrarily in: Group 1 (G1), with the short time of exposure, ≤ 3 years; and Group 2 (G2), with the longer time of exposure, > 3 years. Results: The most relevant complaints were headache (32%) and fatigue (20%), lipothymia (11%), and less commonly sleeplessness and crustiness (both 5%), drowsiness (4%), irritability (3%) and pruritus (1%). 12% of them reported having alcoholic histories. The workers presented concerning alterations found in the blood. Polycythemia (5/88), leukocytosis (10/88) and anemia (19/88) were the most frequent. Hepatic enzymatic damage showed an increase in LDH, bilirubin and AST. Anemia was not associated with the higher LDH rate. Micronucleus (MN) and comet tests were determined in erythrocytes and leukocytes, respectively. The MN test was significant for the total workers group (P = 0.034). As for the class of the comet tails, they were significantly higher for G1 (P = 0.001) and for the total of workers (P = 0.001), for the G2 the significance was borderline (P = 0.05). Conclusion: There were important clinical and laboratorial complaints. Genotoxicity assays indicated DNA damage and they can be useful to prevent serious diseases in this group.

Result(s)* In total 283 patients were included, 185 were classified as NOP while 98 as OP. No differences were found in disease characteristics. Laparoscopy was performed in 82% of patients. Both pelvic and para-aortic lymphadenectomy were performed more frequently in NOP than in OP (56,8% vs. 36,7%; p<0,01 and 45,9 vs. 22,9%; p<0,01). Rates of intra-operative (6,5% vs 12,4%, p=0,12) and post-operative (13,5% vs 20,6% p=0,13) complications were similar between NOP and OP respectively, as well as the severity of complications according to Clavien-Dindo classification (4,4% vs 7,4% grade III, 0,5% vs 0% grade IV, p=0,51). No differences were found in other surgical and post-operative variables. The 5-year disease-specific survival (DSS) rate was lower in OP (66,9% vs 86,0%, p=0,02). When analyzing only patients who underwent complete staging surgery (107 of the NOP and 35 of the OP), no differences were found in perioperative complications rate. In this subgroup, there were no differences in DSS between NOP and OP (78,1% vs 71,0%, p=0,64). Conclusion* OP do not present a higher rate of perioperative complications compared to NOP. However, they underwent less lymphadenectomies and presented poorer DSS. Considering only patients in whom complete surgery was performed, OP presented similar DSS to NOP, without presenting a higher rate of perioperative complications.