Proteomic profile of saliva and plasma from women with impalpable breast lesions

Delmonico, Lucas; Bravo, Maryah; Silvestre, Rafaele Tavares; Ornellas, Maria Helena; Azevedo, Carolina Maria de; Alves, Gilda

doi:10.3892/ol.2016.4828

Cited by 18 publications

(13 citation statements)

References 46 publications

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“…In this report, serum and salivary proteomes showed changes in bitches with CMT when compared to healthy individuals. Some of the proteins that were modulated in the present study such as S100A4 or haptoglobin were also described in previous studies on canine mammary tumors [27] or human breast cancer [14,15,28,29], while other proteins including immunoglobulin gamma-heavy chains A and D or kallikrein-1 were described here for the first time as CMT-related proteins.…”

Section: Discussionsupporting

confidence: 54%

“…In serum, higher levels of histone deacetylase proteins were detected in women with recurrent breast cancer in comparison to non-recurrent cases [13]. In saliva, Delmonico et al [14] identified differences in alpha-2-macroglobulin, ceruplasmin, haptoglobin, hemopexin and vitamin D-binding, among others, in women with impalpable breast cancer in comparison to healthy women; and Giri et al [15] identified 166 proteins with different expression in saliva of women with breast cancer in comparison to healthy controls [15]. In dogs, two studies were reported in which 2-DE gel proteomic analyses of canine mammary tumor tissues were performed.…”

Section: Introductionmentioning

confidence: 96%

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Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Franco‐Martínez

Gelemanović

Horvatić

et al. 2020

Animals

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The aim of this study was to evaluate changes in serum and saliva proteomes in canine mammary tumors (CMT) using a high-throughput quantitative proteomic analysis in order to potentially discover possible biomarkers of this disease. Proteomes of paired serum and saliva samples from healthy controls (HC group, n = 5) and bitches with CMT (CMT group, n = 5) were analysed using a Tandem Mass Tags-based approach. Twenty-five dogs were used to validate serum albumin as a candidate biomarker in an independent sample set. The proteomic analysis quantified 379 and 730 proteins in serum and saliva, respectively. Of those, 35 proteins in serum and 49 in saliva were differentially represented. The verification of albumin in serum was in concordance with the proteomic data, showing lower levels in CMT when compared to the HC group. Some of the modulated proteins found in the present study such as haptoglobin or S100A4 have been related to CMT or human breast cancer previously, while others such as kallikrein-1 and immunoglobulin gamma-heavy chains A and D are described here for the first time. Our results indicate that saliva and serum proteomes can reflect physiopathological changes that occur in CMT in dogs and can be a potential source of biomarkers of the disease.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 96%

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Franco‐Martínez

Gelemanović

Horvatić

et al. 2020

Animals

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“…A study carried out by nano-HPLC-Q-TOF MS investigated the proteome profiles of plasma and saliva of patients with fibroadenoma (n = 10), infiltrating ductal carcinoma (n = 10) and healthy controls (n = 8). The major differentially expressed proteins in the saliva of patients compared with controls were α2-macroglobulin and ceruloplasmin, which should be further validated as potential biomarkers of impalpable breast lesions 30 . A differential proteomic analysis using tandem mass tags technology was performed to characterise potential salivary biomarkers for gastric cancer detection.…”

Section: Human Saliva As Diagnostic Body Fluidmentioning

confidence: 99%

Salivary biomarkers and proteomics: future diagnostic and clinical utilities

Castagnola

Scarano

Passàli

et al. 2017

Acta Otorhinolaryngol Ital

135

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Lo studio della proteomica salivare, test economico e non invasivo, rappresenta una fonte di numerose informazioni, ed è utile per la diagnosi di svariate malattie. Da quando siamo entrati nellera della tecnologia genomica e delle scienze omiche, la raccolta di campioni salivari è aumentata esponenzialmente. Recenti piattaforme proteomiche hanno analizzato il proteoma salivare umano, caratterizzando circa 3000 peptidi e proteine, espressi in maniera differente: più del 90% in peso deriva dalla secrezione delle tre ghiandole salivari maggiori, mentre la restante parte proviene dalle ghiandole salivari minori, dal fluido crevicolare gengivale, da essudati mucosi e dalla microflora orale. Lobiettivo principale dellanalisi proteomica è discriminare tra condizioni fisiologiche e patologiche. Ad oggi, tuttavia, non esiste un preciso protocollo che permetta di analizzare lintero proteoma salivare, pertanto sono state realizzate svariate strategie. Innanzitutto, è possibile distinguere due tipologie di piattaforme proteomiche: lapproccio top-down prevede lanalisi delle proteine sotto esame come entità intatte; nellapproccio bottom-up la caratterizzazione della proteina avviene mediante lo studio dei peptidi ottenuti dopo digestione enzimatica (con tripsina tipicamente). A causa di questa eterogeneità, per una stessa patologia sono stati proposti differenti biomarkers. Il proteoma salivare è stato caratterizzato in numerose malattie: carcinoma squamoso e leucoplachie orali, malattia del trapianto contro lospite (GVHD) cronica, sindrome di Sjögren e altri disordini autoimmuni come la sindrome SAPHO (sinovite, acne, pustolosi, iperostosi e osteite), schizofrenia e disordine bipolare, malattie genetiche come la sindrome di Down o la malattia di Wilson. In conclusione, i risultati delle ricerche riportate in questa review suggeriscono che nel prossimo futuro la saliva diverrà un fluido di indubbia rilevanza diagnostica utile per fini clinici, sia diagnostici, sia prognostici.

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“…The relationship between vitamin D levels and age presented significant association only for the control group (P=0.002). For this reason, according to the ROC plot area (0.778) of the control group, a cut-off value of 45 years old for women was determined, with specificity and sensitivity of 60% and 50%, respectively (Figure 1 flight (nLC-Q-TOF) technology was used, and vitamin D-binding protein was found down expressed in the plasma of cases with benign and malignant breast lesions in comparison with controls´ plasmas (Delmonico et al, 2016). In view of these findings, vitamin D-binding protein should be validated as a putative biomarker in a future study.…”

Section: Discussionmentioning

confidence: 99%

Low Levels of Vitamin D in a Cohort of Women with Impalpable Breast Lesions from Rio de Janeiro/Brazil

Delmonico

Costa²,

Azevedo

et al. 2018

Asian Pac J Cancer Prev

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Background:Low levels of vitamin D have been described as a risk factor for the development of breast cancer. The aim of this study was to evaluate the serum levels of vitamin D (25OHD) in patients with impalpable breast lesions comparing with a control group.Methods:Vitamin D quantification (25OHD) was assessed in the plasma of 65 patients with impalpable breast lesions and from 20 health controls using a chemiluminescent microparticle immunoassay. Pearson’s chi-square test and nonparametric t-Student were used to evaluate statistical significance between the clinical variables and the means of quantification of vitamin D. The receiver operating characteristic (ROC) curve was used to evaluate the correlation between age and vitamin sufficiency for the cases and the controls.Results:The prevalence of vitamin D deficiency and/or insufficiency in women with malignant lesions was 84% and 60% for the control group. Using the chi-square or Fisher’s exact test, the relationship between vitamin D levels and age presented significant association only for the control group (P=0.002). Using ROC curve, the plot area (0.778) for the control group defined a cut-off value of 45 years to age, with specificity and sensitivity of 60% and 50%, respectively. Thus, the odds ratio for vitamin D insufficiency in women over 45 years was 1.37 (P=0.011). For the case group, clinical characteristics, histological grade, and lymph node involvement did not show any significant association.Conclusion:The prevalence of vitamin D deficiency/insufficiency is high in women with impalpable breast lesions, as well as in the control group, even in a tropical city. According to the results the age advancement may be involved with the decrease in vitamin D levels in plasma, but there was no statistical association between low levels of Vitamin D and breast cancer.

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Proteomic profile of saliva and plasma from women with impalpable breast lesions

Cited by 18 publications

References 46 publications

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

Salivary biomarkers and proteomics: future diagnostic and clinical utilities

Low Levels of Vitamin D in a Cohort of Women with Impalpable Breast Lesions from Rio de Janeiro/Brazil

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