Background The nucleotide excision repair (NER) system is one of the most important deoxyribonucleic acid (DNA) repair mechanisms and is critical for chemotherapy resistance. We conducted the present study to investigate the association between two polymorphisms of excision of repair cross-complementing group 1 (ERCC1), the key component of the NER pathway, and the clinicopathological features of patients with non-small cell lung cancer (NSCLC). Methods A total of 38 patients with confirmed NSCLC were included in our study. DNA was extracted from peripheral blood. ERCC1 rs3212986 (8092) and rs11615 (118) were genotyped using molecular assays including polymerase chain reaction (PCR) with restriction fragment length polymorphism (by MboII and HpyCH4 enzymes) and sequencing. Results The PCR results indicated the correct performance of the genomics extraction and molecular protocols. The distribution of C/C, C/A and A/A genotypes at position 8092 was 42.10%, 47.36%, and 10.52% respectively (P=0.03). Multivariate regression analysis showed that there was a significant correlation between C8092A (rs3212986) polymorphism and metastasis, grade of the tumor, and response to treatment. Individuals carrying the rs3212986 CA genotype and A allele had a significantly worse response to the treatment. Also, the correlation between alteration at this genomics location and patients with NSCLC who used to smoke cigarettes was positive. However, no significant association was detected between rs11615 C118>T polymorphism and demographic characteristics of patients with NSCLC. Conclusion We concluded that in lung cancer patients there is a relationship between tumor stage and rs3212986C>A polymorphism.

BACKGROUND:The nucleotide changes in different genetic loci increased the incidence risk of breast cancer.AIM:The aim of present study was to investigate genotype distribution at codon 72 of the TP53 gene (rs1042522) in breast cancer patients to achieve a potential diagnostic marker related to some demographic feathers.METHODS:In our case-control study, blood samples were collected from a total of 34 patients harboured breast cancer. DNA was extracted, and nested-PCR was performed. Products were digested with AccII and subsequently were sequenced. Results were compared with samples characteristics.RESULTS:The PCR results indicated the correct implementation of extraction and amplification protocol. The genotypic distribution at codon 72 of TP53 in control group was 20%, 62.4% and 16.6% for Arg (wildtype), Arg/Pro (heterozygous) and Pro (homozygous variant) respectively. Also, this distribution in the patient group was 23.52% homozygous, 50% heterozygous, and 26.47% another homozygous variant (Adjusted odds ratio: 1.12 and 95%CI = 0.57 to 2.2, P = 0.03). The absence of Arg at codon 72 of TP53 is relevant with age higher than 40 years and metastasis to other organs.CONCLUSION:Polymorphism at codon 72 of TP53 was associated with high-grades of breast cancer risk and different responses to chemotherapy treatment. It is recommended genotype distribution of codon 72 of TP53 before chemotherapy.

Background: Methadone maintenance therapy (MMT) is currently widely accepted as the treatment of choice for opioid replacement therapy. Objectives: The aim of this study was to determine the prevalence of HBV, HCV, and HIV infections as well as psychiatric disorders in our MMT cases. Patients and Methods: Using a cross-sectional study design, we studied patients treated for opioid abuse or addiction who attended MMT programs in Rafsanjan, Iran, from March to June 2012. HIV, HBV, and HCV test reports were recorded from MMT clients' documents (ELISA and Western blotting results), and psychiatric disorders were diagnosed by two trained psychology interviewers according to the DSM-IV criteria. Results: Of the 1,825 patients included in this study, 1,737 (95.2%) were men with a mean age of 28.00 ± 7.69 years (range: 18-52 years). In total, 64 (3.5%) subjects showed symptoms of depression and 21 (1.2%) had anxiety. The prevalence of HBV, HCV, and HIV were 4 (0.2%), 31 (1.7%), and 18 (1.0%), respectively. The association between psychiatric disorders and chronic diseases did not reach a statistically significant level (Fisher's exact test, P = 0.193). Conclusions: The prevalence of HBV, HIV, and psychiatric disorders in our study group was lower than in previous reports. This may be because we entered participants from private MMT centers. Therefore, our results are not generalizable to other populations. Nevertheless, HCV prevalence was higher, and our results lead us to recommend that training programs be planned to prevent the outbreak of these viral infections.

Covid-19 pandemic forced the governments to choose the policy to the lockdown the gathering centers , including universities and schools to prevent the rapid spread of the disease, forcing millions of people to stay at home for several weeks and even more. Staying in the home for such long periods is associated with a change in the wake/sleep period (a kind of social jet lag) and the resulting alteration in feeding time and type of food. Moreover, the prevalence of anxiety and depression resulted from the reduction in physical activity and bad news also may help to worsen the social jetlag effect on these parts of society. Possible hazardous changes in this factor are reviewed in this study and some suggestions for better management of this condition are also offered. Pubmed, SCOPUS, WOS, and Google Scholar data banks were searched using the following keyword in combination and/or alone: jetlag, social jetlag, Chronobiology, photoperiod, metabolic disease, cardiovascular, sleep/weak period. Longtime lockdown gathering centers are shown to change the photoperiod cycle in people, and it may induce several consequences, including the effects on cognitive brain function, induce mental illness, metabolic syndrome, insulin resistance, mitochondrial aging, and cardiovascular diseases. Considering these facts, it is important to make rehabilitation programs for all the societies' members for post-pandemic time.