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Few studies have been reported on the glucose clearance (retention) at different sites on the tooth surfaces. The present study was undertaken to examine the glucose retention (clearance) in the central incisor and first molar of human mouths in 23 volunteer subjects (mean age; 23.3 yrs). Each subject throughly rinsed the mouth for 15s with 20ml of a 0.5M glucose solution and expectorated gently. Small paper-points were fixed to the mesial, distal, buccal, lingual and of the occlusal tooth surfaces. The amounts of gluccse at 3 min were measured with a suger analyzer.The results were as follows; 1) The glucose concentration was highest at the labial surface and lowest at the lingual surface in the central incisor in both maxilla and mandibulla.2) The glucose concentration was highest at the mesial surface and lowest at the occlusal surface in the first molar in the maxilla, it was highest at the buccal surface and lowest at the lingual surface in the first molar in the mandibulla.It was concluded that there was difference of glucose clearance on tooth surfaces.