Banyaknya aktivitas antropogenik seperti pembuangan limbah industri dan domestik ke perairan menyebabkan terjadinya penurunan kulitas air, seperti halnya di sub DAS Martapura Kabupaten Banjar. Tentunya hal ini  patut diwaspadai mengingat keberadaan limbah akan berdampak terhadap kualitas lingkungan dan pengembangan kapasitas sumber daya dalam konteks pembangunan berkelanjutan. Tujuan penelitian ini adalah mengetahui kualitas air yang terdampak limbah sehingga dapat dijadikan sebagai indikator perencanaan pembangunan berkelanjutan di sub DAS Martapura secara khusus. Lokasi penelitian berada di sub DAS Martapura Kabupaten Banjar dengan 4 titik  sampling berdasarkan keberadaan sumber limbah  yang dominan. Kualitas air sungai diukur berdasarkan parameter fisika dan kimia. Analisis kualitas dan penentuan status mutu air menggunakan metode STORET. Hasilnya adalah (1) Parameter fisika-kimia perairan yang terdampak pencemaran  di  sub DAS  Martapura  Kabupaten  Banjar terutama :  DO, BOD, COD, pH dan suhu pada beberapa stasiun pengamatan nilainya telah melebihi baku mutu air sungai Kelas II menurut PP No. 22 Tahun 2021. (2) Hasil perhitungan status mutu air pada sub DAS Martapura menunjukkan total skor sebesar -18. Kondisi perairan di sub DAS Martapura menunjukkan diperlukannya usaha mitigasi dan adaptasi dampak pencemaran lingkungan yang terintegrasi untuk mendukung program pembangunan berkelanjutan. Kata kunci: Kualitas Air, Metode STORET, Sub DAS Martapura. Numerous anthropogenic activities like industrial and domestic waste disposal into the waters causes decreasing water quality, as is the case in the Martapura sub-watershed, Banjar Regency.  This should be watched out for considering waste will affect environmental quality and resource capacity development in context of sustainable development.The research purpose was to determine the water quality affected by waste, using it as an indicator of sustainable development planning in the Martapura sub-watershed specifically. The research location was the Martapura sub-watershed with 4 sampling points based on the presence of dominant waste source. River water quality was measured based on physical and chemical parameters. Quality analysis and determination of water quality status was done using the STORET method. The results are (1)The physico-chemical parameters of waters affected by pollution in the Martapura sub-watershed, Banjar Regency, especially: DO, BOD, COD, pH and temperature at several observation stations have exceeded the Class II river water quality standard according to Government Regulation No. 22 of 2021. (2)The results of the calculation of water quality status in the Martapura sub-watershed show a total score of-18. Martapura sub-watershed waters condition showed the need for integrated mitigation and adaptation of environmental pollution impacts to support sustainable development programs. Keywords: Martapura Sub-Watershed, STORET Method, Water Quality.