In the current rapidly-growing development of globalization era, Twitter has become one of the most favored social media among people, not to mention students. However, behind its popular use, there found no clear evidence regarding the impact of using the application on users. Therefore, this study will discuss about the influence of Twitter use on students, especially on their learning motivation and mindset. It employs a quantitative research method with explanatory research design, and it involves the 3 rdsemester students majoring at English teaching, IAIN Syekh Nurjati, Cirebon, as the research subject. The main instrument of this research is an online questionnaire via Google form. The result of this study indicated that Twitter influences student's learning motivation and mindset with several factors: making no excessive use in duration, following at least 3 educational accounts, and experiencing and feeling the positive impact of the educational content. Therefore, they feel that it provides a significant effect on their mindset and learning motivation.