Cotylorhiza tuberculata is an important scyphozoan jellyfish producing population blooms in the Mediterranean probably due to pelagic ecosystem's decay. Its gastric cavity can serve as a simple model of microbial-animal digestive associations, yet poorly characterized. Using state-of-the-art metagenomic population binning and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), we show that only four novel clonal phylotypes were consistently associated with multiple jellyfish adults. Two affiliated close to Spiroplasma and Mycoplasma genera, one to chlamydial 'Candidatus Syngnamydia', and one to bacteroidetal Tenacibaculum, and were at least one order of magnitude more abundant than any other bacteria detected. Metabolic modelling predicted an aerobic heterotrophic lifestyle for the chlamydia, which were found intracellularly in Onychodromopsis-like ciliates. The Spiroplasma-like organism was predicted to be an anaerobic fermenter associated to some jellyfish cells, whereas the Tenacibaculum-like as free-living aerobic heterotroph, densely colonizing the mesogleal axis inside the gastric filaments. The association between the jellyfish and its reduced microbiome was close and temporally stable, and possibly related to food digestion and protection from pathogens. Based on the genomic and microscopic data, we propose three candidate taxa: 'Candidatus Syngnamydia medusae', 'Candidatus Medusoplasma mediterranei' and 'Candidatus Tenacibaculum medusae'.

No abstract

This study provides new and additional data on morphology and a phylogenetic analysis of the recently described species Pelagia benovici Piraino, Aglieri, Scorrano & Boero, 2014 from the Northern Adriatic (Mediterranean Sea). Comprehensive morphological analyses of diagnostic characters, of which the most significant are marginal tentacles anatomy, basal pillars, gonad pattern, subgenital ostia and exumbrellar sensory pits, revealed significant differences from the currently known genera Sanderia, Chrysaora and Pelagia in the family Pelagiidae. A phylogenetic analysis of mitochondrial genes (COI, 16S rRNA, 12S rRNA) and nuclear ribosomal genes (28S rRNA, ITS1/ITS2 regions), together with cladistic analysis of morphological characters, positioned Pelagia benovici as a sister taxon with Sanderia malayensis, and both share a common ancestor with Chrysaora hysoscella. Pelagia benovici does not share a direct common ancestor with the genus Pelagia, and thus we propose it should not belong to this genus. Therefore, a new genus Mawia, gen. nov. (Semaeostomeae : Pelagiidae) is described, and Pelagia benovici is renamed as Mawia benovici, comb, nov.

Nematocysts of the scyphozoans Pelagia noctiluca and Rhizostoma pulmo were examined. In R. pulmo 4 types of nematocyst were observed: heterotrichous microbasic euryteles; holotrichous isorhizas; atrichous a-isorhizas; and atrichous a-isorhizas. In P. noctiluca 5 types of nematocyst were seen: heterotrichous microbasic euryteles; heterotrichous isorhizas (previously described as atrichous isorhizas); holotrichous O-isorhizas; atrichous a-isorhizas; and an undescribed type, which in its structure and discharge mechanism resembles microbasic p-mastigophores. The results show, in both P. noctiluca and R. pulmo, a greater variety of nematocysts than described in previous studies.

No abstract