In early 1980’s, the Italian scientific community, together with a number of institutional decision-makers, realized how urgent it was to protect natural and environmental resources. They agreed that an adequate level of scientifically organized knowledge allows the accurate planning and development of environment systems through the management and direction of the effective development process, but without stopping it. Since the special VAZAR1 project was first set up in 1984, as part of the GNDCI-CNR2 scientific context it has been the cardinal center point of Research National Program “Aquifer Vulnerability Assessment”. The problem of groundwater contamination was examined in this program for the very first time in Italy in an organic and extensive manner as a key for forecasting and prevention purposes. The Italian approaches to assessing and mapping groundwater vulnerability to contamination are essentially based on two main methodologies: 1) the GNDCI Basic Method [1,2] a HCS type approach that can be used for any type of Italian hydrogeologic situation, even where there is a limited number of data. A unified legend and symbols are also defined for each hydrogeologic level. 2) The SINTACS method [2,3], a PCSM developed for use prevalently in areas with a good data base coverage. The methodological approaches described in this paper now make up the Italian standard which has been dealt with in the recent very important Italian Law (152/993) and which are now ratified in the national guidelines [4] produced by ANPA, the Italian National Agency for Environment Protection. The methods, besides Italy [5] have been applied in several other Countries [6–10] and others

No abstract

No abstract

No abstract

Abstract:Aluminium (Al) has been investigated as a neurotoxic substance. Al ranks among the potential environmental risk factors for Alzheimer's disease (AD). Epidemiological studies tested the relationship between Al in drinking water and AD, showing a significant correlation between elevated levels of monomeric Al in water and AD, although data to date remain inconclusive with respect to total Al. The aim of this study was to test whether or not Al exacerbates cellular toxicity mediated by the amyloid β (Aβ) peptide. We evaluated the role of Al in modulating programmed cell death (apoptosis) in human cell cultures. We used the osteosarcoma cell line monolayer (SaOs-2) to demonstrate that treatment of SaOs-2 cultures with the Aβ peptide mid-fragment (25 to 35) at nano M, followed by co-incubation with physiological concentrations of aluminium chloride, which release monomeric Al in solution, led to marked expression of caspase 3, but not caspase 9, key markers of the apoptotic process. The same experimental conditions were shown to blunt significantly the proliferative response of normal human peripheral blood mononuclear cells (PBMC) to phytohemagglutinin (PHA) stimulation. Our observations support the hypothesis that Al significantly impairs certain cellular immune responses, and confirm that Al-mediated cell toxicity may play an important role in AD.